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arXiv 2310.02585quant-ph
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利用量子关联显微镜在三维中定位两个亚衍射发射体

Localising two sub-diffraction emitters in 3D using quantum correlation microscopy

Shuo Li, Wenchao Li, Qiang Sun, Bill Moran, Timothy C. Brown, Brant C. Gibson, Andrew D. Greentree

AI总结:

本研究将量子关联显微镜(QCM)拓展至三维定位,通过两阶段最大似然估计器实现单个衍射光斑内两个发射体的无衍射极限三维定位,精度与总检测时间平方根成反比,仅需四个测量位置的Hanbury Brown和Twiss测量即可达成。

摘要:

荧光团定位是确定细胞系统生物学功能的重要环节。量子关联显微镜(QCM)是一种有望实现衍射极限下发射体定位的技术,可用于共聚焦或宽场模式。然而,迄今为止,QCM尚未应用于三维定位问题。本文研究表明,量子关联显微镜可对单个衍射极限光斑内的两个发射体实现无衍射极限的三维定位。通过引入两阶段最大似然估计器,我们的建模显示定位精度与$1/\text{sqrt}(t)$成正比,其中$t$为总检测时间。仅需在四个测量位置进行Hanbury Brown和Twiss测量,利用强度和光子关联即可实现无衍射极限定位。

英文摘要:

The localisation of fluorophores is an important aspect of the determination of the biological function of cellular systems. Quantum correlation microscopy (QCM) is a promising technique for providing diffraction unlimited emitter localisation that can be used with either confocal or widefield modalities. However, so far, QCM has not been applied to three dimensional localisation problems. Here we show that quantum correlation microscopy provides diffraction-unlimited three-dimensional localisation for two emitters within a single diffraction-limited spot. By introducing a two-stage maximum likelihood estimator, our modelling shows that localisation precision scales as $1/\sqrt{t}$ where $t$ is the total detection time. Diffraction unlimited localisation is achieved using both intensity and photon correlation from Hanbury Brown and Twiss measurements at as few as four measurement locations.

机构、总结、中文摘要均由AI分析而来,可能存在错误,请以论文正文为准。

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