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arXiv 2301.03451physics.optics
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无标记非相干超分辨光学显微镜

Label-free incoherent super-resolution optical microscopy

Nikhil Jayakumar, Luis E. Villegas-Hernandez, Weisong Zhao, Hong Mao, Firehun T Dullo, Jean Claude Tinguley, Krizia Sagini, Alicia Llorente, Balpreet Singh Ahluwalia

AI总结:

本文提出 EPSLON 技术,利用氮化硅波导的倏逝衰减光致发光散射实现稳定、强度线性的无标记非相干近场照明,从而支持 SIM 与 IFON 突破远场衍射极限。

摘要:

荧光分子的光动力学已使规避远场光学衍射极限成为可能。尽管其潜力巨大,但对样品进行标记的必要性可能会对所研究的脆弱生物学过程产生不利影响。因此,需要持续开展开发工作,以突破远场无标记衍射障碍。无标记模式下探测光的相干性阻碍了现有基于非相干荧光成像的超分辨方法的应用。在本文中,我们阐述了相关物理原理,并提出一种方法,通过利用氮化硅波导的光致发光对未标记样品进行近场照明来规避这一挑战。该技术缩写为 EPSLON,即“倏逝衰减光致发光散射实现无标记光学纳米显微术”。我们证明,这种照明具有模拟纳米尺寸荧光分子光动力学的特性。这使得开发一种在强度上线性且随时间稳定的无标记非相干系统成为可能,从而允许在无标记模式下应用结构光照明显微镜(SIM)和基于强度涨落的光学纳米显微术(IFON)等技术,以规避衍射极限。

英文摘要:

The photo-kinetics of fluorescent molecules have enabled the circumvention of far-field optical diffraction-limit. Despite its enormous potential, the necessity to label the sample may adversely influence the delicate biology under investigation. Thus, continued development efforts are needed to surpass the far-field label-free diffraction barrier. The coherence of the detected light in label-free mode hinders the application of existing super-resolution methods based on incoherent fluorescence imaging. In this article, we present the physics and propose a methodology to circumvent this challenge by exploiting the photoluminescence of silicon nitride waveguides for near-field illumination of unlabeled samples. The technique is abbreviated EPSLON, Evanescently decaying Photoluminescence Scattering enables Label-free Optical Nanoscopy. We demonstrate that such an illumination has properties that mimic the photo-kinetics of nano-sized fluorescent molecules. This allows for developing a label-free incoherent system that is linear in intensity, and stable with time thereby permitting the application of techniques like structured illumination microscopy (SIM) and intensity-fluctuation-based optical nanoscopy (IFON) in label-free mode to circumvent the diffraction limit.

机构、总结、中文摘要均由AI分析而来,可能存在错误,请以论文正文为准。

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