彩色共焦断层成像
Chromatic Confocal Tomography
摘要：
共聚焦显微镜是世界各地细胞研究实验室的支柱，但不幸的是，成像仅限于单个平面。彩色共焦显微镜提供了同时成像多个平面的可能性，从而提供了成像速度的多方面增加，同时消除了对z轴扫描的需要。标准的彩色共焦系统具有几百微米量级的有限成像范围，这限制了它们的应用。在这项工作中，使用一个单一的硒化锌透镜，我们展示了一个彩色共焦断层扫描系统，具有18毫米的成像范围与平均横向分辨率为2.46微米和另一个系统与1.55毫米的成像范围与0.86微米的平均横向分辨率。这样做，我们已经把彩色共焦系统的成像范围从微米到厘米制度，大约两个数量级的改进。所提出的方法可以是一个强大的工具，共聚焦成像的生物样品或工业样品的表面分析。
英文摘要：
Confocal microscopy is the backbone of cellular research labs across the world but unfortunately, the imaging is restricted to a single plane. Chromatic confocal microscopy offers the possibility to image multiple planes simultaneously thus providing a manifold increase in the imaging speed whereas eliminating the need for z-axis scanning. Standard chromatic confocal systems have a limited imaging range of the order of a few hundreds of micrometers which limits their applications. In this work, using a single zinc selenide lens, we demonstrate a chromatic confocal tomography system that has an imaging range of 18 mm with an average lateral resolution of 2.46 microns and another system with a 1.55 mm imaging range with 0.86 microns average lateral resolution. Doing so, we have pushed the imaging range of chromatic confocal systems from micrometer to centimeter regime, an improvement of approximately two orders of magnitude. The proposed approach can be a powerful tool for confocal imaging of biological samples or surface profiling of industrial samples.
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