作为无标记纳米温度计的含色氨酸蛋白质
Tryptophan-containing proteins as label-free nanothermometers
摘要：
仍然需要以高时空分辨率监测热过程的技术，其在化学、生物学和工程学中具有无数潜在应用。从纳米级分子现象的温度测量是特别有前途的，由于它们与生物系统的一般兼容性。在这些现象中，荧光偏振各向异性是特别有吸引力的，由于其高灵敏度的热和物理信息，以及其广泛的实用性与广泛的荧光光谱。在这项工作中，我们建议，色氨酸的固有荧光偏振各向异性的测量可以用来测量在纳米级的各种含色氨酸的蛋白质的无标记温度。我们提出了一个理论模型的温度敏感性的荧光偏振在游离色氨酸和含色氨酸的蛋白质，经验探索这些属性，并讨论了潜在的发展蛋白质作为热记忆传感器。
英文摘要：
There remains a need for techniques to monitor thermal processes at high spatiotemporal resolution, with myriad potential applications in chemistry, biology, and engineering. Measurement of temperature from nanoscale molecular phenomena are particularly promising due to their general compatibility with biological systems. Among these phenomena, fluorescence polarization anisotropy is particularly attractive due to its high sensitivity to thermal and photophysical information, and its general utility with a wide spectrum of fluorophores. In this work, we propose that the measurement of the intrinsic fluorescence polarization anisotropy of tryptophan can be used to measure label-free temperature at the nanoscale in a variety of tryptophan-containing proteins. We present a theoretical model of the temperature sensitivity of fluorescence polarization in free tryptophan and tryptophan-containing proteins, empirically explore these properties, and discuss the potential development of proteins as thermal memory sensors.
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