A strong non-equilibrium bound for sorting of crosslinkers on growing biopolymers

Weakly nonlinear rheology of transiently crosslinked biopolymer gels

Recent experimental investigations have revealed a non-Maxwellian absorption pattern in the rheological spectra of actin gels, which was interpreted in terms of transient bonds. Here we examine the consequences of reversible crosslinking on the apparent linear spectra of biopolymer solutions theoretically. For a schematic model consisting of a reversibly crosslinked power-law fluid we obtain a simple analytical prediction for the position of the absorption peak, which is backed up by a numerical evaluation of the inelastic glassy wormlike chain model. This establishes bond breaking as a nonlin…

Theory of Crosslinked Bundles of Helical Filaments: Intrinsic Torques in Self-Limiting Biopolymer Assemblies

Inspired by the complex influence of the globular crosslinking proteins on the formation of biofilament bundles in living organisms, we study and analyze a theoretical model for the structure and thermodynamics of bundles of helical filaments assembled in the presence of crosslinking molecules. The helical structure of filaments, a universal feature of biopolymers such as filamentous actin, is shown to generically frustrate the geometry of crosslinking between the "grooves" of two neighboring filaments. We develop a coarse-grained model to investigate the interplay between the geometry of bind…

Non-linear stress relaxation of transient-crosslinked biopolymer networks

A long standing puzzle in the rheology of living cells is the origin of the experimentally observed long time stress relaxation. The mechanics of the cell is largely dictated by the cytoskeleton, which is a biopolymer network consisting of transient crosslinkers, allowing for stress relaxation over time. Moreover, these networks are internally stressed due to the presence of molecular motors. In this work we propose a theoretical model that uses a mode-dependent mobility to describe the stress relaxation of such prestressed transient networks. Our theoretical predictions agree favorably with e…

Strain Stiffening Induced by Molecular Motors in Active Crosslinked Biopolymer Networks

We have studied the elastic response of actin networks with both compliant and rigid crosslinks by modeling molecular motors as force dipoles. Our finite element simulations show that for compliant crosslinkers such as filamin A, the network can be stiffened by two orders of magnitude while stiffening achieved with incompliant linkers such as scruin is significantly smaller, typically a factor of two, in excellent agreement with recent experiments. We show that the differences arise from the fact that the motors are able to stretch the compliant crosslinks to the fullest possible extent, which…

解构细胞骨架交叉连接中的熵、主动和摩擦力

Disentangling Entropic, Active, and Frictional Forces in Cytoskeletal Crosslinking

本文提出了一种理论框架，用于解构细胞骨架交叉连接中熵、主动和摩擦力的相互作用，以解释交叉连接蛋白如何集体产生力。