用于相衬电子显微术的高功率近同心法布里-珀罗腔
High-Power Near-Concentric Fabry-Perot Cavity for Phase Contrast Electron Microscopy
摘要：
玻璃化生物大分子（cryo-EM）的透射电子显微术（TEM）受到可从此类样品获得的弱相衬信号的限制。因此，使用相位板将显著提高信噪比。我们以前已经证明了使用高功率法布里-珀罗腔作为TEM的相位板。我们现在报告对我们的激光腔的改进，使我们能够实现超过450 GW/cm $^{2}$的创纪录连续波强度，足以产生300 keV电子的最佳90°相移。此外，我们已经使用激光相位板进行了第一次冷冻EM重建，证明该激光相位板的稳定性足以在标准冷冻EM数据收集期间使用。
英文摘要：
Transmission electron microscopy (TEM) of vitrified biological macromolecules (cryo-EM) is limited by the weak phase contrast signal that is available from such samples. Using a phase plate would thus substantially improve the signal-to-noise ratio. We have previously demonstrated the use of a high-power Fabry-Perot cavity as a phase plate for TEM. We now report improvements to our laser cavity that allow us to achieve record continuous-wave intensities of over 450 GW/cm$^{2}$, sufficient to produce the optimal 90° phase shift for 300 keV electrons. In addition, we have performed the first cryo-EM reconstruction using a laser phase plate, demonstrating that the stability of this laser phase plate is sufficient for use during standard cryo-EM data collection.
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