远距离聚焦显微镜对放大率失配的灵敏度
Sensitivity of Remote Focusing Microscopes to Magnification Mismatch
摘要：
远程聚焦（RF）是一种技术，大大扩展了光学显微镜的无像差轴向扫描范围。为了使RF系统中的衍射限制深度范围最大化，中继透镜的放大率应该使得物镜的光瞳平面精确地映射到彼此上。在本文中，我们研究了射频系统的容差放大率失配和量化的残余球差量存在于不同的聚焦深度。我们观察到，从理想的放大倍率的小偏差的结果，在剩余的球面像差项导致的动态范围的减少量的增加。对于高数值孔径物镜，模拟预测的1%的放大率失配的动态范围减少了50%。仿真结果已被验证对一个实验的RF系统与理想和非理想的放大率。实验证实的预测还提供了一种有价值的经验方法，根据焦点两侧球面像差的符号来确定系统何时接近理想相位匹配条件。
英文摘要：
Remote focusing (RF) is a technique that greatly extends the aberration-free axial scan range of an optical microscope. To maximise the diffraction limited depth range in an RF system, the magnification of the relay lenses should be such that the pupil planes of the objectives are accurately mapped on to each other. In this paper we study the tolerance of the RF system to magnification mismatch and quantify the amount of residual spherical aberration present at different focusing depths. We observe that small deviations from ideal magnification results in increased amounts of residual spherical aberration terms leading to a reduction in the dynamic range. For high numerical aperture objectives, the simulation predicts a 50% decrease in the dynamic range for 1% magnification mismatch. The simulation has been verified against an experimental RF system with ideal and non-ideal magnifications. Experimentally confirmed predictions also provide a valuable empirical method of determining when a system is close to the ideal phase matching condition, based on the sign of the spherical aberration on either side of focus.
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