使用共振高光谱成像研究早期生物膜的附着和抗生素反应
Attachment and antibiotic response of early-stage biofilms studied using resonant hyperspectral imaging
摘要：
许多细菌物种容易形成生物膜，其充当抵抗外部挑战（例如来自抗微生物处理）的保护性基质。因此，生物膜通常是持续性和复发性感染的原因。研究生物膜的既定方法要么是破坏性的，要么集中在生物膜表面。非常需要对生物膜下侧敏感的非破坏性方法，因为它允许研究抗生素通过膜的渗透。在这里，我们证明了共振高光谱成像的高表面灵敏度提供了这种能力。该方法使我们能够监测大肠杆菌生物膜形成，细胞附着和小菌落形成的早期阶段，原位和实时。我们研究了生物膜对一些不同抗生素的反应，并使用共聚焦显微镜验证了我们的观察结果。基于这种密切监测表面结合细胞的能力，共振高光谱成像为生物膜的抗菌素耐药性提供了新的见解。
英文摘要：
Many bacterial species readily develop biofilms that act as a protective matrix against external challenge, e.g. from antimicrobial treatment. Therefore, biofilms are often responsible for persistent and recurring infections. Established methods for studying biofilms are either destructive or they focus on the biofilm surface. A non-destructive method that is sensitive to the underside of the biofilm is highly desirable, as it allows studying the penetration of antibiotics through the film. Here, we demonstrate that the high surface sensitivity of resonant hyperspectral imaging provides this capability. The method allows us to monitor the early stages of Escherichia coli biofilm formation, cell attachment and microcolony formation, in-situ and in real time. We study the response of the biofilm to a number of different antibiotics and verify our observations using confocal microscopy. Based on this ability to closely monitor the surface-bound cells, resonant hyperspectral imaging gives new insights into the antimicrobial resistance of biofilms.
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