使用X射线laser的纳米颗粒集合体的3D衍射成像
3D diffractive imaging of nanoparticle ensembles using an X-ray laser
摘要：
报道了利用X射线单粒子成像（SPI）技术测定金纳米粒子（AuNPs）的三维结构。利用欧洲X射线自由电子激光的新功能，在不到100小时的光束时间内测量了大约1000万个来自金纳米颗粒的衍射图案，这是之前任何单个SPI实验数据量的100倍以上，该功能允许每秒1500帧的测量。开发了一种分类和结构分选方法，以解开颗粒的异质性，并获得优于3 nm的分辨率。随着这些新的实验和分析的发展，我们已经进入了SPI方法的新时代，生物分子接近原子分辨率成像的道路是显而易见的。
英文摘要：
We report the 3D structure determination of gold nanoparticles (AuNPs) by X-ray single particle imaging (SPI). Around 10 million diffraction patterns from gold nanoparticles were measured in less than 100 hours of beam time, more than 100 times the amount of data in any single prior SPI experiment, using the new capabilities of the European X-ray free electron laser which allow measurements of 1500 frames per second. A classification and structural sorting method was developed to disentangle the heterogeneity of the particles and to obtain a resolution of better than 3 nm. With these new experimental and analytical developments, we have entered a new era for the SPI method and the path towards close-to-atomic resolution imaging of biomolecules is apparent.
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