Developing angular trapping methods, which will enable optical tweezers to rotate a micronized bead, is of great importance for the studies of biomacromolecules during a wide range of torque-generation processes. Here we report a novel controlled angular trapping method based on composite Janus particles. We used a chemically synthesized Janus particle, which consists of two hemispheres made of polystyrene (PS) and poly(methyl methacrylate) (PMMA) respectively, as a model system to demonstrate this method. Through computational and experimental studies, we demonstrated the feasibility to control the rotation of a Janus particle in a linearly polarized laser trap. Our results showed that the Janus particle aligned its two hemisphere's interface parallel to the laser propagation direction as well as the laser polarization direction. In our experiments, the rotational state of the particle can be easily and directly visualized by using a CMOS camera, and does not require complex optical detection system. The rotation of the Janus particle in the laser trap can be fully controlled in real time by controlling the laser polarization direction. Our newly developed angular trapping technique has the great advantage of easy implementation and real time controllability. Considering the easy chemical synthesis of Janus particles and implementation of the angular trapping, this novel method has the potential of becoming a general angular trapping method. We anticipate that this new method will significantly broaden the availability of angular trapping in the biophysics community, and expand the scope of the research that can be enabled by the angular trapping approach.
We present a new, high-speed technique to track the three-dimensional translation and rotation of non-spherical colloidal particles. We capture digital holograms of micrometer-scale silica rods and sub-micrometer-scale Janus particles freely diffusing in water, and then fit numerical scattering models based on the discrete dipole approximation to the measured holograms. This inverse-scattering approach allows us to extract the the position and orientation of the particles as a function of time, along with static parameters including the size, shape, and refractive index. The best-fit sizes and…
Analytical solution for optical trapping force on a spherical dielectric particle for an arbitrary positioned focused beam is presented in a generalized Lorenz-Mie and vectorial diffraction theory. In this case the exact electromagnetic field is considered in the focal region. A double tweezers setup was employed to perform ultra sensitive force spectroscopy and observe the forces, demonstrating the selectively couple of the transverse electric (TE), transverse magnetic (TM) modes by means of the beam polarization and positioning, and to observe correspondent morphology-dependent resonances (M…
We fabricate a miniature spherical mirror for tightly focusing an optical dipole trap for neutral atoms. The mirror formation process is modelled to predict the dimensions for particular fabrication parameters. We integrate the spherical mirror with a neutral atom experiment to trap and detect a single atom with high efficiency. The mirror serves the dual purpose of focusing the dipole trap as well as collection of the atomic fluorescence into an optical fibre.
Optical trapping, where microscopic particles are trapped and manipulated by light is a powerful and widespread technique, with the single-beam gradient trap (also known as optical tweezers) in use for a large number of biological and other applications. The forces and torques acting on a trapped particle result from the transfer of momentum and angular momentum from the trapping beam to the particle. Despite the apparent simplicity of a laser trap, with a single particle in a single beam, exact calculation of the optical forces and torques acting on particles is difficult. Calculations can be…
Optical trapping can be used to manipulate the three-dimensional (3-D) motion of spherical particles based on the simple prediction of optical forces and the responding motion of samples. However, controlling the 3-D behaviour of non-spherical particles with arbitrary orientations is extremely challenging, due to experimental difficulties and the extensive computations. Here, we achieved the real-time optical control of arbitrarily shaped particles by combining the wavefront shaping of a trapping beam and measurements of the 3-D refractive index (RI) distribution of samples. Engineering the 3-…
Optical trapping and manipulation using laser beams play a key role in many areas including biology, atomic science, and nanofabrication. Here, we propose and experimentally demonstrate the first use of a vortex-pair beam in optical trapping and manipulation. We successfully trap two spherical microparticles simultaneously by a single vortex-pair beam. Precisely position-controllable manipulation of the trapped spherical microparticles is realized by adjusting the off-axis distance of the vortices on the initial phase plane of the vortex-pair beam. Based on the feature of the vortex-pair beam,…
Experimental observations suggest that there are differences between the behavior of particles optically trapped in air and trapped in a liquid phase. We present a modified version of Mie Debye Spherical Aberration theory to numerically simulate such optical system in attempt to explain and predict these effects. The model incorporates Mie scattering and focussing of the trapping beam through media of stratified refractive index. Our results show a geometrical optics approach cannot correctly describe our system and that spherical aberration must be included. We successfully qualitatively expl…
Arthur Ashkin was awarded the 2018 Nobel prize in physics for the invention of optical tweezers. Since the first publication in 1986 Optical Tweezers have been used as a tool to measure forces and rheological properties of microscopic systems. For the calibration of these measurements, the knowledge of the forces is fundamental. However, it is still common to deduce the optical forces from assumptions based on the particle size with respect to the trapping laser wavelength. This show the necessity to develop a complete and accurate electromagnetic model that does not depend on early approximat…
Computationally modeling the behavior of wavelength-sized non-spherical particles in optical tweezers can give insight into the existence and stability of trapping equilibria as well as the optical manipulation of such particles more broadly. Here, we report Brownian dynamics simulations of non-spherical particles that account for detailed optical, hydrodynamic, and thermal interactions. We use a $T$-matrix formalism to calculate the optical forces and torques exerted by focused laser beams on clusters of wavelength-sized spheres, and we incorporate detailed diffusion tensors that capture the …
It was theoretically proposed and experimentally demonstrated that anti-reflection coating allows one to trap a high dielectric sphere, at the same time enhancing the transverse optical force. Here, by explicitly calculating the gradient force and the scattering force, we rigorously show that these were mainly consequences of the reduction in scattering force due to the suppression of backward scattering, and enhancement in gradient force due to the increased in overall particle size. The reduction of scattering force can be understood within a ray optics theory and also the Mie theory. The co…
We use the T-matrix formalism in combination with the method of far-field matching to evaluate the optical force exerted by Laguerre-Gaussian (LG) light beams on a spherical (Mie) particle. For both non-vortex and optical vortex LG beams, the theoretical results are used to analyze the optical-force-induced dynamics of the scatterer near the trapping points represented by the equilibrium (zero-force) positions. The regimes of linearized dynamics are described in terms of the stiffness matrix spectrum and the damping constant of the ambient medium. For the purely azimuthal LG beams, the dynamic…
Light scattering by small particles has a long and interesting history in physics. Nonetheless, it continues to surprise with new insights and applications. This includes new discoveries, such as novel plasmonic effects, as well as exciting theoretical and experimental developments such as optical trapping, anomalous light scattering, optical tweezers, nano-spasers, and novel aspects and realizations of Fano resonances. These have led to important new applications, including several ones in the biomedical area and in sensing techniques at the single-molecule level. There are additionally many …
We observe the opto-mechanical multistability of a macroscopic torsion balance oscillator. The torsion oscillator forms the moving mirror of a hemi-spherical laser light cavity. When a laser beam is coupled into this cavity, the radiation pressure force of the intra-cavity beam adds to the torsion wire's restoring force, forming an opto-mechanical potential. In the absence of optical damping, up to 23 stable trapping regions were observed due to local light potential minima over a range of 4 micrometer oscillator displacement. Each of these trapping positions exhibits optical spring properties…
Focused laser beams allow controlling mechanical motion of objects and can serve as a tool for assembling complex micro and nano structures in space. While in a vast majority of cases small particles experience attractive gradient forces and repulsive radiation pressure, introduction of additional degrees of freedom into optomechanical manipulation suggests approaching new capabilities. Here we analyze optical forces acting on a high refractive index silicon sphere in a focused Gaussian beam and reveal new regimes of particles anti-trapping. Multipolar analysis allows separating an optical for…
Non-invasive optical manipulation of particles has emerged as a powerful and versatile tool for biological study and nanotechnology. In particular, trapping and rotation of cells, cell nuclei and sub-micron particles enables unique functionality for various applications such as tissue engineering, cancer research and nanofabrication. We propose and demonstrate a purely optical approach to rotate and align particles using the interaction of polarized light with photonic crystal nanostructures to generate enhanced trapping force. With a weakly focused laser beam we observed efficient trapping an…
We demonstrate an optical system that can apply and accurately measure the torque exerted by the trapping beam on a rotating birefringent probe particle. This allows the viscosity and surface effects within liquid media to be measured quantitatively on a micron-size scale using a trapped rotating spherical probe particle. We use the system to measure the viscosity inside a prototype cellular structure.
