用于测量培养基中心肌收缩力的高耐用裂纹传感器与硅橡胶悬臂梁集成
Highly durable crack sensor integrated with silicone rubber cantilever for measuring cardiac contractility in culture media
摘要：
我们提出了一种新的悬臂梁装置集成了聚二甲基硅氧烷（PDMS）封装的裂纹传感器，直接测量心肌收缩力。裂纹传感器被化学键合到PDMS薄层以形成夹层结构，其允许在培养基中非常稳定地操作。与没有封装层相比，所提出的裂纹传感器的可靠性显著提高。在评估了与PDMS层结合的裂纹传感器的耐久性之后，在纳米图案化的悬臂梁上培养心肌细胞以实时测量心脏收缩力。高灵敏度裂纹传感器连续测量心脏收缩力，而不改变其测量因子长达26天（> 500万次心跳）。此外，由药物引起的收缩力的变化进行了监测，使用裂纹传感器集成悬臂。最后，将实验结果与传统电生理方法的结果进行比较，验证了建立基于收缩的药物毒性测试系统的可行性。
英文摘要：
We propose a novel cantilever device integrated with a polydimethylsiloxane (PDMS)-encapsulated crack sensor that directly measures the cardiac contractility. The crack sensor was chemically bonded to a PDMS thin layer to form a sandwiched structure which allows to be operated very stably in culture media. The reliability of the proposed crack sensor has improved dramatically compared to no encapsulation layer. After evaluating the durability of the crack sensor bonded with the PDMS layer, cardiomyocytes were cultured on the nano-patterned cantilever for real-time measurement of cardiac contractile forces. The highly sensitive crack sensor continuously measured the cardiac contractility without changing its gauge factor for up to 26 days (>5 million heartbeats). In addition, changes in contractile force induced by drugs were monitored using the crack sensor-integrated cantilever. Finally, experimental results were compared with those obtained via conventional electrophysiological methods to verify the feasibility of building a contraction-based drug-toxicity testing system.
机构、总结、中文摘要均由AI分析而来，可能存在错误，请以论文正文为准。
