In this paper, we describe how to apply image-to-image translation techniques to medical blood smear data to generate new data samples and meaningfully increase small datasets. Specifically, given the segmentation mask of the microscopy image, we are able to generate photorealistic images of blood cells which are further used alongside real data during the network training for segmentation and object detection tasks. This image data generation approach is based on conditional generative adversarial networks which have proven capabilities to high-quality image synthesis. In addition to synthesizing blood images, we synthesize segmentation mask as well which leads to a diverse variety of generated samples. The effectiveness of the technique is thoroughly analyzed and quantified through a number of experiments on a manually collected and annotated dataset of blood smear taken under a microscope.
The application of machine learning techniques to the medical domain is especially challenging due to the required level of precision and the incurrence of huge risks of minute errors. Employing these techniques to a more complex subdomain of hematological diagnosis seems quite promising, with automatic identification of blood cell types, which can help in detection of hematologic disorders. In this paper, we benchmark 27 popular deep convolutional neural network architectures on the microscopic peripheral blood cell images dataset. The dataset is publicly available, with large number of norma…
Leukemia (blood cancer) is an unusual spread of White Blood Cells or Leukocytes (WBCs) in the bone marrow and blood. Pathologists can diagnose leukemia by looking at a person's blood sample under a microscope. They identify and categorize leukemia by counting various blood cells and morphological features. This technique is time-consuming for the prediction of leukemia. The pathologist's professional skills and experiences may be affecting this procedure, too. In computer vision, traditional machine learning and deep learning techniques are practical roadmaps that increase the accuracy and spe…
Leukemia is diagnosed with complete blood counts which is by calculating all blood cells and compare the number of white blood cells (White Blood Cells / WBC) and red blood cells (Red Blood Cells / RBC). Information obtained from a complete blood count, has become a cornerstone in the hematology laboratory for diagnostic purposes and monitoring of hematological disorders. However, the traditional procedure for counting blood cells manually requires effort and a long time, therefore this method is one of the most expensive routine tests in laboratory hematology clinic. Solution for such kind of…
Robust White Blood Cell Classification with Stain-Normalized Decoupled Learning and Ensembling
University of Technology, Ho Chi Minh City, Vietnam（越南胡志明市技术大学）；University of Science, Ho Chi Minh City, Vietnam（越南胡志明市科学大学）；Vietnam National University, Ho Chi Minh City, Vietnam（越南国家大学）；Carnegie Mellon University, Pittsburgh, PA, USA（卡内基梅隆大学）；Northwestern University, Chicago, IL, USA（西北大学）
Effectively determining malaria parasitemia is a critical aspect in assisting clinicians to accurately determine the severity of the disease and provide quality treatment. Microscopy applied to thick smear blood smears is the de facto method for malaria parasitemia determination. However, manual quantification of parasitemia is time consuming, laborious and requires considerable trained expertise which is particularly inadequate in highly endemic and low resourced areas. This study presents an end-to-end approach for localisation and count of malaria parasites and white blood cells (WBCs) whic…
School of Electrical and Information Engineering, Tianjin University（天津大学电气与信息工程学院）；The Hang Seng University of Hong Kong（香港恒生大学）
This paper proposes a novel automatic classification framework for the recognition of five types of white blood cells. Segmenting complete white blood cells from blood smears images and extracting advantageous features from them remain challenging tasks in the classification of white blood cells. Therefore, we present an adaptive threshold segmentation method to deal with blood smears images with non-uniform color and uneven illumination, which is designed based on color space information and threshold segmentation. Subsequently, after successfully separating the white blood cell from the bloo…
creating automated processes in different areas of medical science with the application of engineering tools is a highly growing field over recent decades. In this context, many medical image processing and analyzing researchers use worthwhile methods in artificial intelligence, which can reduce necessary human power while increases accuracy of results. Among various medical images, blood microscopic images play a vital role in heart failure diagnosis, e.g., blood cancers. The prominent component in blood cancer diagnosis is white blood cells (WBCs) which due to its general characteristics in …
Indian Institute of Information Technology Allahabad（印度信息技术学院阿拉哈巴德）；Universitas Budi Luhur（布迪鲁胡尔大学）
Machine learning has endless applications in the health care industry. White blood cell classification is one of the interesting and promising area of research. The classification of the white blood cells plays an important part in the medical diagnosis. In practise white blood cell classification is performed by the haematologist by taking a small smear of blood and careful examination under the microscope. The current procedures to identify the white blood cell subtype is more time taking and error-prone. The computer aided detection and diagnosis of the white blood cells tend to avoid the h…
Federal University of São Carlos（圣卡洛斯联邦大学）；University of Ottawa（渥太华大学）；Ottawa Hospital Research Institute（渥太华医院研究所）
Characterizing blood vessels in digital images is important for the diagnosis of many types of diseases as well as for assisting current researches regarding vascular systems. The automated analysis of blood vessels typically requires the identification, or segmentation, of the blood vessels in an image or a set of images, which is usually a challenging task. Convolutional Neural Networks (CNNs) have been shown to provide excellent results regarding the segmentation of blood vessels. One important aspect of CNNs is that they can be trained on large amounts of data and then be made available, f…
This paper presents an effective color normalization method for thin blood film images of peripheral blood specimens. Thin blood film images can easily be separated to foreground (cell) and background (plasma) parts. The color of the plasma region is used to estimate and reduce the differences arising from different illumination conditions. A second stage normalization based on the database-gray world algorithm transforms the color of the foreground objects to match a reference color character. The quantitative experiments demonstrate the effectiveness of the method and its advantages against …
University of Bristol（布里斯托大学）；Chulalongkorn University（朱拉隆功大学）
Identification of abnormalities in red blood cells (RBC) is key to diagnosing a range of medical conditions from anaemia to liver disease. Currently this is done manually, a time-consuming and subjective process. This paper presents an automated process utilising the advantages of machine learning to increase capacity and standardisation of cell abnormality detection, and its performance is analysed. Three different machine learning technologies were used: a Support Vector Machine (SVM), a classical machine learning technology; TabNet, a deep learning architecture for tabular data; U-Net, a se…
BloodCell-Net: A lightweight convolutional neural network for the classification of all microscopic blood cell images of the human body
Khulna University of Engineering and Technology（库尔纳工程技术大学）；Bangladesh Atomic Energy Regulatory Authority（孟加拉国原子能监管局）；Zarqa University（扎尔卡大学）；Northumbria University（诺森比亚大学）；Bangabandhu Sheikh Mujibur Rahman Science and Technology University（班加班杜谢赫穆吉布尔·拉赫曼科技大学）；Northern Border University（北方边境大学）
Retinal vessel segmentation is a fundamental step in screening, diagnosis, and treatment of various cardiovascular and ophthalmic diseases. Robustness is one of the most critical requirements for practical utilization, since the test images may be captured using different fundus cameras, or be affected by various pathological changes. We investigate this problem from a data augmentation perspective, with the merits of no additional training data or inference time. In this paper, we propose two new data augmentation modules, namely, channel-wise random Gamma correction and channel-wise random v…
Classification of White Blood Cells Using Machine and Deep Learning Models: A Systematic Review
University of Lahore（拉合尔大学）；University of Limerick（利默里克大学）；Dublin Institute of Technology（都柏林理工学院）；National University of Sciences and Technology（国家科技大学）
Institute for Design Problems in Microelectronics of Russian Academy of Sciences（俄罗斯科学院微电子设计问题研究所）；Shenzhen Research Institute of Big Data（深圳大数据研究院）；University of Michigan（密歇根大学）；Cork University Hospital（科克大学医院）
Adequate blood supply is critical for normal brain function. Brain vasculature dysfunctions such as stalled blood flow in cerebral capillaries are associated with cognitive decline and pathogenesis in Alzheimer's disease. Recent advances in imaging technology enabled generation of high-quality 3D images that can be used to visualize stalled blood vessels. However, localization of stalled vessels in 3D images is often required as the first step for downstream analysis, which can be tedious, time-consuming and error-prone, when done manually. Here, we describe a deep learning-based approach for …
Carotid artery vessel wall thickness measurement is an essential step in the monitoring of patients with atherosclerosis. This requires accurate segmentation of the vessel wall, i.e., the region between an artery's lumen and outer wall, in black-blood magnetic resonance (MR) images. Commonly used convolutional neural networks (CNNs) for semantic segmentation are suboptimal for this task as their use does not guarantee a contiguous ring-shaped segmentation. Instead, in this work, we cast vessel wall segmentation as a multi-task regression problem in a polar coordinate system. For each carotid a…
Existing methods to reconstruct vascular structures from a computed tomography (CT) angiogram rely on injection of intravenous contrast to enhance the radio-density within the vessel lumen. However, pathological changes can be present in the blood lumen, vessel wall or a combination of both that prevent accurate reconstruction. In the example of aortic aneurysmal disease, a blood clot or thrombus adherent to the aortic wall within the expanding aneurysmal sac is present in 70-80% of cases. These deformations prevent the automatic extraction of vital clinically relevant information by current m…
Diagnosis Support of Sickle Cell Anemia by Classifying Red Blood Cell Shape in Peripheral Blood Images
Facultad de Ciencias Naturales y Exactas, Universidad de Oriente（自然科学与精确科学系，奥里ente大学）；Balearic Islands Health Research Institute (IdISBa)（巴利阿里群岛健康研究 institutes（IdISBa））；Department of Mathematics and Computer Science, Universitat de les Illes Balears（数学与计算机科学系，巴利阿里群岛大学）；Research Institute of Health Sciences (IUNICS)（健康科学研究院（IUNICS））；Computational biology and bioinformatics (BIOCOM) Research Group（计算生物学和生物信息学（BIOCOM）研究组）
Leading University（领先大学）；Clemson University（克莱姆森大学）
We apply convolutional neural networks to identify between malaria infected and non-infected segmented cells from the thin blood smear slide images. We optimize our model to find over 95% accuracy in malaria cell detection. We also apply Canny image processing to reduce training file size while maintaining comparable accuracy (~ 94%).
Southwest Minzu University（西南民族大学）；West China Biomedical Big Data Center（华西生物医学大数据中心）
Blood cell detection in microscopic images is an essential branch of medical image processing research. Since disease detection based on manual checking of blood cells is time-consuming and full of errors, testing of blood cells using object detectors with Deep Convolutional Neural Network can be regarded as a feasible solution. In this work, an object detector based on YOLOF has been proposed to detect blood cell objects such as red blood cells, white blood cells and platelets. This object detector is called TE-YOLOF, Tiny and Efficient YOLOF, and it is a One-Stage detector using dilated enco…
A novel and fast semi-automatic method for segmentation, locating and counting blood cells in an image is proposed. In this method, thresholding is used to separate the nucleus from the other parts. We also use Hough transform for circles to locate the center of white cells. Locating and counting of red cells is performed using template matching. We make use of finding local maxima, labeling and mean value computation in order to shrink the areas obtained after applying Hough transform or template matching, to a single pixel as representative of location of each region. The proposed method is …
ITER, Siksha ‘O’ Anusandhan (Deemed to be University)（ITER，锡克沙·奥·阿努桑德汉（被视为大学））
In the medical domain, different computer-aided diagnosis systems have been proposed to extract blood vessels from retinal fundus images for the clinical treatment of vascular diseases. Accurate extraction of blood vessels from the fundus images using a computer-generated method can help the clinician to produce timely and accurate reports for the patient suffering from these diseases. In this article, we integrate top-hat based preprocessing approach with fine-tuned B-COSFIRE filter to achieve more accurate segregation of blood vessel pixels from the background. The use of top-hat transformat…
