利用工程化点扩散函数进行高速扩展容积血流测量
High-speed extended-volume blood flow measurement using engineered point-spread function
摘要：
生物体中血流的实验表征对于理解心血管系统的发育和功能至关重要，但是还没有报道用于以高精度对大体积厚样品进行快照成像的技术。我们结合了计算显微镜和无衍射，自弯曲属性的艾里光束跟踪荧光珠与亚微米精度通过扩展的轴向范围（高达600 $μ$ m）内的流动血液受精后3天（DPF）斑马鱼胚胎。示踪珠在流动的血液中的空间轨迹被记录在运输过程中通过两个主和intersegmentated血管，和轨迹被发现是一致的使用选择性平面照明显微镜（SPIM）记录的脉管系统的分割。这种方法提供了精确的空间和时间测量的三维血流的基础动力学，如壁剪切应力或心血管疾病的测量增强的理解的潜力。
英文摘要：
Experimental characterization of blood flow in living organisms is crucial for understanding the development and function of cardiovascular systems, but there have been no techniques reported for snapshot imaging of thick samples in large volumes with high precision. We have combined computational microscopy and the diffraction-free, self-bending property of Airy beams to track fluorescent beads with sub-micron precision through an extended axial range (up to 600 $μ$m) within the flowing blood of 3 days post-fertilization (dpf) zebrafish embryos. The spatial trajectories of the tracer beads within flowing blood were recorded during transit through both cardinal and intersegmental vessels, and the trajectories were found to be consistent with the segmentation of the vasculature recorded using selective-plane illumination microscopy (SPIM). This method provides precise spatial and temporal measurement of 3D blood flow with potential for enhanced understanding of fundamental dynamics, such as measurement of wall shear stress or of cardiovascular disease.
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