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arXiv 2012.11892eess.IVcs.CVcs.LGphysics.optics

Deep learning-based virtual refocusing of images using an engineered point-spread function

University of California, Los Angeles(加利福尼亚大学洛杉矶分校)

Xilin Yang, Luzhe Huang, Yilin Luo, Yichen Wu, Hongda Wang, Yair Rivenson, Aydogan Ozcan

英文摘要:

We present a virtual image refocusing method over an extended depth of field (DOF) enabled by cascaded neural networks and a double-helix point-spread function (DH-PSF). This network model, referred to as W-Net, is composed of two cascaded generator and discriminator network pairs. The first generator network learns to virtually refocus an input image onto a user-defined plane, while the second generator learns to perform a cross-modality image transformation, improving the lateral resolution of the output image. Using this W-Net model with DH-PSF engineering, we extend the DOF of a fluorescence microscope by ~20-fold. This approach can be applied to develop deep learning-enabled image reconstruction methods for localization microscopy techniques that utilize engineered PSFs to improve their imaging performance, including spatial resolution and volumetric imaging throughput.

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