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arXiv 1802.03007physics.optics
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无传感器自适应光学用于布里渊显微光谱学

Sensor-less adaptive optics for Brillouin micro-spectroscopy

Eitan Edrei, Giuliano Scarcelli

摘要:

布里渊光谱是一种非接触式粘弹性表征的强大光学技术。然而,像所有的光学系统,布里渊光谱性能退化的光学像差,并因此被限制到均匀的透明样品。为了校正像差,自适应光学(AO)方法先前已经被集成到从地面望远镜到超分辨率显微镜的各种光学模态中。在这项工作中,我们开发了一个自适应光学配置设计的布里渊散射光谱分析。我们的配置不需要直接的波前传感和“引导星”的注入;因此,它可以在不需要样品预处理的情况下实现。我们使用我们的AO-布里渊光谱仪在像差幻影和生物样品;与以前的AO系统一致,我们证明了有效的校正光学像差产生2.5倍的布里渊信号强度增强和1.4倍的轴向分辨率提高。

英文摘要:

Brillouin spectroscopy is a powerful optical technique for viscoelastic characterization of samples without contact. However, like all optical systems, Brillouin spectroscopy performances are degraded by optical aberrations, and have therefore been limited to homogenous transparent samples. To correct for aberrations, adaptive optics (AO) methods have been previously integrated into a variety of optical modalities ranging from ground-based telescopes to super-resolution microscopes. In this work, we developed an adaptive optics configuration designed for Brillouin scattering spectral analysis. Our configuration doesn't require direct wave-front sensing and the injection of a 'guide-star'; hence, it can be implemented without the need for sample pre-treatment. We used our AO-Brillouin spectrometer in aberrated phantoms and biological samples; consistent with previous AO systems, we demonstrate effective correction of optical aberrations yielding 2.5-fold enhancement in Brillouin signal strength and 1.4-fold improvement in axial resolution.

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