DNA在α-溶血素纳米孔中的转位及其在大分子数据存储中的潜在应用
DNA translocation through alpha-haemolysin nano-pores with potential application to macromolecular data storage
摘要：
数字信息可以被编码在大分子（如RNA和单链DNA）的构建块序列中。“写入”和“读取”大分子链的方法目前是可用的，但它们缓慢且昂贵。在理想的分子数据存储系统中，诸如写入、读取、擦除、存储和传输的常规操作必须在集成芯片内可靠地且高速地完成。作为证明这一概念的可行性的第一步，我们报告了在小型化的腔室中进行的DNA读出实验的初步结果，这些腔室可以扩展到更小的尺寸。我们表明，易位的单链DNA分子（由50个腺苷碱基，随后由100个胞嘧啶碱基组成）通过离子通道产生的特征信号，这是由于分子的2段结构。我们还研究了实验的可调参数的易位率和速度的依赖性。
英文摘要：
Digital information can be encoded in the building-block sequence of macromolecules, such as RNA and single-stranded DNA. Methods of "writing" and "reading" macromolecular strands are currently available, but they are slow and expensive. In an ideal molecular data storage system, routine operations such as write, read, erase, store, and transfer must be done reliably and at high speed within an integrated chip. As a first step toward demonstrating the feasibility of this concept, we report preliminary results of DNA readout experiments conducted in miniaturized chambers that are scalable to even smaller dimensions. We show that translocation of a single-stranded DNA molecule (consisting of 50 adenosine bases followed by 100 cytosine bases) through an ion-channel yields a characteristic signal that is attributable to the 2-segment structure of the molecule. We also examine the dependence of the translocation rate and speed on the adjustable parameters of the experiment.
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