立足点介导的RNA链置换的建模
Modelling toehold-mediated RNA strand displacement
摘要：
我们研究的RNA支点介导的链置换反应的热力学和动力学与最近开发的粗粒度的RNA模型。链置换，在此期间单链置换先前结合到互补底物链的不同链，是活性核酸纳米技术中的基本机制，并且也被假设在体内发生。我们研究了位移反应的速率作为立足点和温度的长度的函数，并做出两个实验可检验的预测：如果立足点放置在基板的5'端，位移会更快，并且随着温度的升高，位移会减慢。
英文摘要：
We study the thermodynamics and kinetics of an RNA toehold-mediated strand displacement reaction with a recently developed coarse-grained model of RNA. Strand displacement, during which a single strand displaces a different strand previously bound to a complementary substrate strand, is an essential mechanism in active nucleic acid nanotechnology and has also been hypothesized to occur in vivo. We study the rate of displacement reactions as a function of the length of the toehold and temperature and make two experimentally testable predictions: that the displacement is faster if the toehold is placed at the 5' end of the substrate and that the displacement slows down with increasing temperature for longer toeholds.
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