通过脂质双层之间的去湿实现快速膜半融合
Fast Membranes Hemifusion via Dewetting between Lipid Bilayers
摘要：
脂双层的行为对于理解细胞的功能性（如细胞之间的离子运输）非常重要。探索脂质双层和半融合状态的性质的标准程序通常使用支撑膜或囊泡。这两种技术在生物相关性或测量的可访问性方面都有几个缺点。在这篇文章中，使用优化的微流控方案，允许同时进行光学和电生理测量模型细胞膜之间的独立半融合状态的形成进行了研究。在第一步骤中，使用液滴界面双层（DiB）技术的变型在微流体装置内的期望位置处形成两个模型膜。在第二步骤中，使两个模型膜接触，形成单个半熔融状态。对于所有测试的脂质，独立膜之间的半融合状态在数百毫秒内形成，即比文献中报道的快几个数量级。半熔融态的形成是一个两阶段的过程，而第二阶段可以解释为一个去湿过程中的无滑移边界条件。所形成的半融合状态是长寿命的，并且当由所施加的电场触发时可以观察到单个融合事件，如对于单油酸甘油酯所证明的。
英文摘要：
The behavior of lipid bilayer is important to understand the functionality of cells like the trafficking of ions between cells. Standard procedures to explore the properties of lipid bilayer and hemifused states typically use either supported membranes or vesicles. Both techniques have several shortcoming in terms of bio relevance or accessibility for measurements. In this article the formation of individual free standing hemifused states between model cell membranes is studied using an optimized microfluidic scheme which allows for simultaneous optical and electrophysiological measurements. In a first step, two model membranes are formed at a desired location within a microfluidic device using a variation of the droplet interface bilayer (DiB) technique. In a second step, the two model membranes are brought into contact forming a single hemifused state. For all tested lipids, the hemifused state between free standing membranes form within hundreds of milliseconds, i.e. several orders of magnitude faster than reported in literature. The formation of a hemifused state is observed as a two stage process, whereas the second stage can be explained as a dewetting process in no-slip boundary condition. The formed hemifusion states are long living and a single fusion event can be observed when triggered by an applied electric field as demonstrated for monoolein.
机构、总结、中文摘要均由AI分析而来，可能存在错误，请以论文正文为准。
