arXiv 1408.52162014-08-22q-bio.QM

非均匀DNA复制动力学的数值模拟

Numerical modeling of inhomogeneous DNA replication kinetics

Michel G. Gauthier, Antoine Dubé, John Bechhoefer

备注：: This paper was written in 2011 as a chapter for a book project that, ultimately, failed to attract enough chapters to be published. We have not updated the references or discussion to take into account the significant amount of work done in this field since then. See, for example, A. Baker and J. Bechhoefer, arxiv1312.4590 / Phys. Rev. E 89, 032703 (2014) for more recent developments

摘要：

我们提出了一种计算技术建模不均匀的DNA复制动力学，复制因素，如起始速率或叉速度可以随位置和时间而变化。我们可以使用我们的模型来模拟通过分子梳理获得的数据集，分子梳理是一种广泛使用的探测复制的实验技术。我们还可以通过将我们的模型拟合到实验数据集来推断有关复制程序的信息，并通过将我们的模型拟合到模拟数据集来测试计划实验的有效性。我们认为异步数据集，并说明如何缺乏同步影响复制配置文件。除了梳理数据，我们的技术非常适合基于微阵列的复制研究。

英文摘要：

We present a calculation technique for modeling inhomogeneous DNA replication kinetics, where replication factors such as initiation rates or fork speeds can change with both position and time. We can use our model to simulate data sets obtained by molecular combing, a widely used experimental technique for probing replication. We can also infer information about the replication program by fitting our model to experimental data sets and also test the efficacy of planned experiments by fitting our model to simulated data sets. We consider asynchronous data sets and illustrate how a lack of synchrony affects replication profiles. In addition to combing data, our technique is well-adapted to microarray-based studies of replication.

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摘要：

英文摘要：

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