基于氧化铝荧光核径迹探测器的离子径迹三维重建
Ion track reconstruction in 3D using alumina-based fluorescent nuclear track detectors
摘要：
基于Al2O3：C，Mg单晶的荧光核径迹探测器（FNTDs）结合共聚焦显微镜提供了离子径迹的三维信息，其分辨率仅受光衍射的限制。FNTD也是用细胞涂覆的理想基底，以设计细胞荧光离子轨道混合检测器。这种放射生物学工具能够在质子和重离子治疗中在亚细胞水平上将细胞反应与物理剂量沉积联系起来。为了实现细胞涂层中的单个离子撞击与其生物响应之间的空间相关性，必须使用由FNTD读出获得的深度信息在3D中重建离子穿越。FNTD用汇合的人肺腺癌上皮细胞层包被。混合检测器的碳离子照射垂直于检测器表面和与检测器表面成角度地进行。荧光标记的细胞层和FNTD的原位成像在顺序读出中进行。利用FNTD提供的轨迹信息，研究了单粒子穿越混合探测器的三维轨迹重建精度。精确度受到照射角度的强烈影响，因此受到FNTD信号复杂性的强烈影响。周向照射的精度最高，误差小于0.10°。FNTD技术提供精确的3D离子径迹重建的能力使其成为临床离子束中放射生物学研究的有力工具，无论是用作涂覆活组织的基底还是植入体内。
英文摘要：
Fluorescent nuclear track detectors (FNTDs) based on Al2O3:C,Mg single crystal combined with confocal microscopy provide 3D information on ion tracks with a resolution only limited by light diffraction. FNTDs are also ideal substrates to be coated with cells to engineer cell-fluorescent ion track hybrid detectors. This radiobiological tool enables a novel platform linking cell responses to physical dose deposition on a sub-cellular level in proton and heavy ion therapies. To achieve spatial correlation between single ion hits in the cell coating and its biological response the ion traversals have to be reconstructed in 3D using the depth information gained by the FNTD read-out. FNTDs were coated with a confluent human lung adenocarcinoma epithelial cell layer. Carbon ion irradiation of the hybrid detector was performed perpendicular and angular to the detector surface. In-situ imaging of the fluorescently labeled cell layer and the FNTD was performed in a sequential read-out. Making use of the trajectory information provided by the FNTD the accuracy of 3D track reconstruction of single particles traversing the hybrid detector was studied. The accuracy is strongly influenced by the irradiation angle and therefore by complexity of the FNTD signal. Perpendicular irradiation results in highest accuracy with error of smaller than 0.10°. The ability of FNTD technology to provide accurate 3D ion track reconstruction makes it a powerful tool for radiobiological investigations in clinical ion beams, either being used as a substrate to be coated with living tissue or being implanted in vivo.
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