碳纳米管网络固定葡萄糖氧化酶
Glucose oxidase immobilization onto carbon nanotube networking
摘要：
在制备基于单壁碳纳米管（SWNTs）的生物传感器时，必须解决目标生物分子在纳米管表面的固定化这一重要问题。在这项工作中，酶（葡萄糖氧化酶（GOX））被固定在纳米管网络的表面上，这是通过喷雾法从1，2-二氯苯溶液中沉积纳米管而产生的。1-芘丁酸琥珀酰亚胺酯（PSE）被用来形成分子界面，其双功能分子提供与酶壳的共价结合，其另一部分（芘）被吸附到纳米管表面。首先，这种分子界面的使用排除了酶（在这种情况下，其活性降低）在纳米管表面上的直接吸附，其次，它确保了酶在纳米管附近的定位。原始纳米管的共振拉曼（RR）光谱与其在PSE环境中的光谱的比较证明了由SWNT与PSE分子形成的纳米混合物的产生，这提供了进一步的酶固定化。由于SWNT：PSE：GOX膜的RR光谱与SWNT：PSE膜的RR光谱没有本质上的不同，这表明分子界面（PSE）足够强地将酶与纳米管隔离。由于PSE沿碳纳米管的GOX的有效固定化证实与原子力显微镜图像。分子动力学的方法使我们能够建立在水环境中创建的单壁碳纳米管：PSE：GOX的结构，并确定杂化组件之间的相互作用能。此外，单壁碳纳米管网络的导电性与吸附PSE和GOX分子进行了研究。
英文摘要：
When elaborating the biosensor based on single-walled carbon nanotubes (SWNTs), it is necessary to solve such an important problem as the immobilization of a target biomolecule on the nanotube surface. In this work, the enzyme (glucose oxidase (GOX)) was immobilized on the surface of a nanotube network, which was created by the deposition of nanotubes from their solution in 1,2-dichlorobenzene by the spray method. 1-Pyrenebutanoic acid succinimide ester (PSE) was used to form the molecular interface, the bifunctional molecule of which provides the covalent binding with the enzyme shell, and its other part (pyrene) is adsorbed onto the nanotube surface. First, the usage of such a molecular interface leaves out the direct adsorption of the enzyme (in this case, its activity decreases) onto the nanotube surface, and, second, it ensures the enzyme localization near the nanotube. The comparison of the resonance Raman (RR) spectrum of pristine nanotubes with their spectrum in the PSE environment evidences the creation of a nanohybrid formed by an SWNT with a PSE molecule which provides the further enzyme immobilization. As the RR spectrum of an SWNT:PSE:GOX film does not essentially differ from that of SWNT:PSE ones, this indicates that the molecular interface (PSE) isolates the enzyme from nanotubes strongly enough. The efficient immobilization of GOX along the carbon nanotubes due to PSE is confirmed with atom-force microscopy images. The method of molecular dynamics allowed us to establish the structures of SWNT:PSE:GOX created in the aqueous environment and to determine the interaction energy between hybrid components. In addition, the conductivity of the SWNT network with adsorbed PSE and GOX molecules is studied.
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