\textit{Vibrio harveyi}和\textit{Vibrio cholerae}群体感应诱导发光表型差异的计算机模拟
Computational modeling of differences in the quorum sensing induced luminescence phenotypes of \textit{Vibrio harveyi} and \textit{Vibrio cholerae}
摘要：
\textit{Vibrio harveyi}和\textit{Vibrio cholerae}具有设计相似的群体感应途径和高度同源的组分，包括多种小RNA（sRNA）。然而，sRNA缺失菌株的相关发光表型显著不同：在\textit{V. harveyi}中，sRNA起相加作用;然而，在\textit{V. cholerae}中，sRNA起冗余作用。此外，\textit{V. harveyi}和\textit{V. cholerae}中不同途径突变体的发光表型存在显著差异。然而，这些差异与在这些细菌中观察到的sRNA缺失菌株的差异无关。在这项工作中，我们提出了一个模型的群体感应诱导发光表型集中在多个sRNA与靶mRNA的相互作用。在我们的模型中，我们发现一个关键参数--发光激活所需的蛋白质浓度的倍数变化--可以控制sRNAs是否表现出相加或冗余的作用。对于特定的参数选择，我们发现，在这个关键参数的差异也可以解释迄今为止未连接的发光表型差异的各种途径突变体的\textit{V. harveyi}和\textit{V. cholerae}。因此，该模型可以提供一个统一的解释观察到的发光表型的差异，也可以用来为未来的实验进行可测试的预测。
英文摘要：
\textit{Vibrio harveyi} and \textit{Vibrio cholerae} have quorum sensing pathways with similar design and highly homologous components including multiple small RNAs (sRNAs). However, the associated luminescence phenotypes of strains with sRNA deletions differ dramatically: in \textit{V. harveyi}, the sRNAs act additively; however, in \textit{V. cholerae}, the sRNAs act redundantly. Furthermore, there are striking differences in the luminescence phenotypes for different pathway mutants in \textit{V. harveyi} and \textit{V. cholerae}. However these differences have not been connected with the observed differences for the sRNA deletion strains in these bacteria. In this work, we present a model for quorum sensing induced luminescence phenotypes focusing on the interactions of multiple sRNAs with target mRNA. Within our model, we find that one key parameter -- the fold-change in protein concentration necessary for luminescence activation -- can control whether the sRNAs appear to act additively or redundantly. For specific parameter choices, we find that differences in this key parameter can also explain hitherto unconnected luminescence phenotypes differences for various pathway mutants in \textit{V. harveyi} and \textit{V. cholerae}. The model can thus provide a unifying explanation for observed differences in luminescence phenotypes and can also be used to make testable predictions for future experiments.
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