FTIR光谱成像作为超声作用于体外细胞的探针
FTIR spectral imaging as a probe of ultrasound effect on cells in vitro
摘要：
基因或药物的安全和有效的细胞内递送在靶向癌症治疗和基因治疗应用中至关重要。超声波（US）已被证明可以改变细胞膜的通透性，由于生物物理机制（声致穿孔），并开发作为一种有前途的非侵入性基因转移方法。声穿孔过程可以诱导瞬时孔的形成，而不显著影响细胞活力。本研究旨在研究治疗性超声（脉冲-1 MHz）的一些生物效应，这些生物效应可以增强药物或基因在非肿瘤细胞系中的传递。我们使用NIH-3 T3细胞系作为模型系统，并暴露于US在两个不同的距离，从源;这种脉冲超声波对细胞的影响进行了评估，通过傅立叶变换红外（FT-IR）光谱成像分析。这种技术结合焦平面阵列（FPA）检测器已被广泛用于研究一般的生物化学变化在体外;此外，FPA检测器的发展和缩短测量时间专门用于红外成像，从几个小时到几分钟，已经有可能图像的分子物种在生物样品中的分布。我们还进行了一项分裂阻滞微核（CBMN）试验，以揭示是否存在由于DNA损伤而在细胞分裂期间形成的微核（称为Howell-Jolly小体）。IR分析结合细胞遗传学分析结果表明，这些实验条件不会引起NIH-3 T3细胞系的DNA突变。最后，从光谱图中提取的平均光谱的光谱参数与来自光谱图的所有光谱的集合的光谱参数之间的比较可能受到图内坏像素的存在的限制。
英文摘要：
Safe and efficient intracellular delivery of genes or drugs is critically important in targeted cancer treatment and gene therapy applications. Ultrasound (US) has been demonstrated to alter the cell membrane permeability due to a biophysical mechanism (Sonoporation) and exploited as a promising non-invasive gene transfer method. The sonoporation process could induce the formation of transient pores without significantly affecting cell viability. This research is aimed at investigating some bioeffects due to Therapeutic Ultrasound (pulsed-1 MHz) which could allow to enhance drugs or genes delivery in a non tumoral cell line. We have used the NIH-3T3 cell line as model system and exposed it to US at two different distances from the source; the effects of this pulsed ultrasonic wave on cells were assessed by Fourier transform infrared (FT-IR) spectroscopic imaging analysis. This technique combined with a focal plane array (FPA) detector has been widely used to study the general biochemical changes in vitro; moreover, the development of FPA detectors and shortening of measurement times specifically for IR imaging, from several hours to few minutes, have made possible to image the distribution of molecular species in biological samples. We have also performed a cytokinesis-block micronucleus (CBMN) assay to reveal the presence or not of micronuclei (named Howell-Jolly bodies) formed during the cell division due to the DNA damage. The results of IR analysis combined with the cytogenetic analysis have shown that these experimental conditions can not cause DNA mutations in the NIH-3T3 cell line. Finally, the comparison between the spectral parameters of the average spectrum extracted from the spectral map and those of the set of all spectra from the spectral map could be limited by the presence of bad pixels inside the map.
机构、总结、中文摘要均由AI分析而来，可能存在错误，请以论文正文为准。
