聚电解质与带相反电荷的蛋白质静电复合物的反离子释放：直接测量
Counterions release from electrostatic complexes of polyelectrolytes and proteins of opposite charge : a direct measurement
摘要：
虽然经常被认为是相反电荷的物种的络合的主要驱动过程之一，但反离子的释放从未在实验上直接测量过对蛋白质/蛋白质复合物的影响。我们在这里提出的第一个结构确定这样的释放由小角中子散射在复合物制成的溶菌酶，带正电荷的蛋白质和PSS，带负电荷的蛋白质。这两种组分具有相同的中子密度长度，因此它们的散射可以在适当的“匹配”溶剂中同时关闭;这使得能够确定复合物内单个抗衡离子的空间分布。反离子（包括经受曼宁缩合的反离子）从核中排出，其中物质处于静电化学计量。
英文摘要：
Though often considered as one of the main driving process of the complexation of species of opposite charges, the release of counterions has never been experimentally directly measured on polyelectrolyte/proteins complexes. We present here the first structural determination of such a release by Small Angle Neutron Scattering in complexes made of lysozyme, a positively charged protein and of PSS, a negatively charged polyelectrolyte. Both components have the same neutron density length, so their scattering can be switched off simultaneously in an appropriate "matching" solvent; this enables determination of the spatial distribution of the single counterions within the complexes. The counterions (including the one subjected to Manning condensation) are expelled from the cores where the species are at electrostatic stoichiometry.
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