驱动蛋白与克鲁克斯涨落定理

驱动蛋白热力学性能中的能量转换、波动抑制和信息传递

Energy Conversion, Fluctuation Suppression, and Information Transfer in the Thermodynamic Performance of Kinesin

研究驱动蛋白热力学性能，通过构建两态模型，推导并比较多种效率的解析表达式与模拟结果，发现其效率低，说明仅热力学效率不能完全表征驱动蛋白性能，需进一步研究互补动力学观点。

Unraveling on Kinesin Acceleration in Intracellular Environments: A Theory for Active Bath

Single molecular motor kinesin harnesses thermal and non-thermal fluctuations to transport various cargoes along microtubules, converting chemical energy to directed movements. To describe the non-thermal fluctuations generated by the complex environment in living cells, we establish a bottom-up model to mimic the intracellular environment, by introducing an active bath consisting of active Ornstein-Uhlenbeck (OU) particles. Simulations of the model system show that kinesin and the probe attached to it are accelerated by such active bath. Further, we provide a theoretical insight into the simu…

Noise-induced acceleration of single molecule kinesin-1

The movement of single kinesin molecules was observed while applying noisy external forces that mimic intracellular active fluctuations. We found kinesin accelerates under noise, especially when a large hindering load is added. The behavior quantitatively conformed to a theoretical model that describes the kinesin movement with simple two-state reactions. The universality of the kinetic theory suggests that intracellular enzymes share a similar noise-induced acceleration mechanism, i.e. active fluctuations in cells are not just noise but are utilized to promote various physiological processes.

Application of the fluctuation theorem to motor proteins: from F1-ATPase to axonal cargo transport by kinesin and dynein

The fluctuation theorem is a representative theorem in non-equilibrium statistical physics actively studied in the 1990's. Relating to entropy production in non-equilibrium states, the theorem has been used to estimate the driving power of motor proteins from fluctuation in their motion. In this review, usage of the fluctuation theorem in experiments on motor proteins is illustrated for biologists, especially those who study mechanobiology, in which force measurement is a central issue. We first introduce the application of the fluctuation theorem in measuring the rotary torque of the rotary m…

驱动蛋白马达动力学的结构视角

A structural perspective on the dynamics of kinesin motors

尽管在热噪声下会有显著的波动，但细胞中的生物机器仍能以极高的精度执行任务。使用粗粒度模型的分子模拟和理论论证，我们设想了驱动蛋白，生物机器的原型，如何产生力，并调节其动力学，以维持持续的运动动作。基于结构的模型，它可以是通用的，在适应其结构的外部压力，同时保持其原生的折叠，被用来解释几个功能的驱动蛋白动力学沿生化循环。这种分析补充了我们目前对驱动蛋白动力学的理解和实验的联系。我们提出了一个热力学循环的驱动蛋白，强调机械和调节作用的颈部连接器和澄清有关的问题的电机方向性，和外部失速力和内部张力之间的差异负责的头-头协调。驱动蛋白和宏观热机的热力学循环之间的比较突出了结构变化作为生物分子机器中工作产生的来源的重要性。

涨落定理与自由能恢复的新进展

Recent progress in fluctuation theorems and free energy recovery

本文综述了近年来有关涨落关系及其在单分子实验中自由能恢复的应用研究进展。我们强调的重要性的业务定义的机械工作和波动关系下的非不变性伽利略变换，这两个方面目前适合实验测试。最后，我们描述了一个推广的Crooks波动关系有用的恢复自由能的部分平衡状态和热力学分支。