用于细胞生物学的单个自体荧光蛋白的荧光显微术
Fluorescence microscopy of single autofluorescent proteins for cellular biology
摘要：
本文综述了自体荧光蛋白在单分子生物学成像中的应用。绿色荧光蛋白（GFP）和DsRed的几个突变体的物理特性进行了比较，并严格讨论其在细胞生物学中的应用。在单分子水平或荧光相关光谱的双光子激发的替代使用被设想用于个体自体荧光蛋白的研究。单分子实验中进行的活细胞使用eGFP，最好是eYFP融合蛋白进行审查。最后，第一次使用在单分子水平的黄嘌呤，一个更光稳定的变体的eYFP时，融合到一个受体的神经递质在活细胞中的报告。
英文摘要：
In this paper we review the applicability of autofluorescent proteins for single-molecule imaging in biology. The photophysical characteristics of several mutants of the Green Fluorescent Protein (GFP) and those of DsRed are compared and critically discussed for their use in cellular biology. The alternative use of two-photon excitation at the single-molecule level or Fluorescence Correlation Spectroscopy is envisaged for the study of individual autofluorescent proteins. Single-molecule experiments performed in live cells using eGFP and preferably eYFP fusion proteins are reviewed. Finally, the first use at the single-molecule level of citrine, a more photostable variant of the eYFP is reported when fused to a receptor for neurotransmitter in live cells.
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