具有永久扭结的DNA的熵弹性
Entropic elasticity of DNA with a permanent kink
摘要：
许多蛋白质与细胞中的双链DNA相互作用并使其变形。单分子实验已经研究了DNA与螺旋变形蛋白质的弹性，包括弯曲DNA的蛋白质。这些实验增加了对包括螺旋变形蛋白质在内的DNA弹性理论的需求。以前关于弯曲DNA的理论工作已经研究了具有许多非特异性结合蛋白的长DNA分子。然而，最近的实验使用相对较短的DNA分子，具有单一的，明确定义的弯曲位点。在这里，我们开发一个简单的理论描述的影响，一个单一的弯曲。然后，我们包括在有限蠕虫状链模型（FWLC）的短DNA分子连接到珠的弯曲的描述。我们预测如何DNA力的延伸关系的变化，由于形成一个单一的永久性扭结，在所有值的施加拉伸力。我们的预测表明，高分辨率的单分子实验可以确定蛋白质结合时诱导的弯曲角。
英文摘要：
Many proteins interact with and deform double-stranded DNA in cells. Single-molecule experiments have studied the elasticity of DNA with helix-deforming proteins, including proteins that bend DNA. These experiments increase the need for theories of DNA elasticity which include helix-deforming proteins. Previous theoretical work on bent DNA has examined a long DNA molecule with many nonspecifically binding proteins. However, recent experiments used relatively short DNA molecules with a single, well-defined bend site. Here we develop a simple theoretical description of the effect of a single bend. We then include the description of the bend in the finite worm like chain model (FWLC) of short DNA molecules attached to beads. We predict how the DNA force-extension relation changes due to formation of a single permanent kink, at all values of the applied stretching force. Our predictions show that high-resolution single-molecule experiments could determine the bend angle induced upon protein binding.
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