Hydrosomes：用于单分子研究和微流体的光学捕获毫微微升容器
Hydrosomes: Optically trapped femtoliter containers for single molecule studies and microfluidics
摘要：
我们展示了一种新的技术，用于创建，操纵和结合毫微微升体积的化学容器。可能的用途包括创建涉及少量试剂的受控化学反应，以及通过荧光成像技术研究容器内单个分子的动力学。容器，我们称之为水相体，是表面活性剂稳定的低折射率氟碳介质中的水液滴。容器和碳氟化合物之间的折射率失配使得单个水质体可以被单焦点激光束（即光学镊子）光学捕获。在这里我们捕获并操纵单个的水质体。我们证明了一个可控的化学反应，融合的水相体含有DNA片段长度约1000碱基对的水相体含有YOYO-1，DNA嵌入染料。我们进一步检测来自水小体中的单个染料分子的荧光，并观察来自连接到单链16聚体DNA分子的Cy 3-Cy 5分子的单对荧光共振能量转移（spFRET）。
英文摘要：
We demonstrate a novel technique for creating, manipulating, and combining femtoliter volume chemical containers. Possible uses include creating controlled chemical reactions involving small quantities of reagent, and studying the dynamics of single molecules within the containers by fluorescence imaging techniques. The containers, which we call hydrosomes, are surfactant stabilized aqueous droplets in a low index-of-refraction fluorocarbon medium. The index of refraction mismatch between the container and fluorocarbon is such that individual hydrosomes can be optically trapped by single focus laser beams, i.e. optical tweezers. Here we trap and manipulate individual hydrosomes. We demonstrate a controlled chemical reaction by the fusion of a hydrosome containing DNA segments approximately 1000 base pairs in length with a hydrosome containing YOYO-1, a DNA intercalating dye. We furthermore detect the fluorescence from single dye molecules in a hydrosome, and observe single pair fluorescence resonance energy transfer (spFRET) from Cy3-Cy5 molecules attached to a single-stranded 16mer DNA molecule.
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