用荧光染料吲哚菁监测掺入细胞色素c氧化酶的蛋白脂质体膜电位
Monitoring of the Membrane Potential in Proteoliposomes with Incorporated Cytochrome-c Oxidase Using the Fluorescent Dye Indocyanine
摘要：
提出了一种实时监测囊泡膜电位变化的方法。利用电位敏感的荧光染料吲哚菁，在水/脂质再分配模型的基础上，提出了一种估算掺入细胞色素C氧化酶的囊泡膜电位的计算方法。物理参数，如囊泡的大小分布和密度的脂质双层进行了估计，并作为计算参数。通过将瞬态电位变化外推到零时，计算了电位变化的初始速率（dU/dt）。它还表明，初始电位变化（dU/dt）可用于研究质子/电子化学计量的细胞色素-c氧化酶纳入囊泡。
英文摘要：
A method has been developed to monitor changes of the membrane potential across vesicle membranes in real time. Using the potential-sensitive fluorescent dye indocyanine and on the basis of water/lipid redistribution model, a calculation procedure has been introduced to estimate the membrane potential in vesicles with incorporated cytochrome-c oxidase. Physical parameters, such as vesicle size distribution and density of the lipid bilayer were estimated and used as calculation parameters. By extrapolation of transient potential change to zero time, the initial rate of the potential change (dU/dt) has been calculated. It is also shown, that the initial potential change (dU/dt) may be used to study the proton/electron stoichiometry of cytochrome-c oxidase incorporated in the vesicles.
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