糖萼驱动的微囊泡形成动力学

中文摘要 AI 辅助

微囊泡（MVs）是一种由细胞分泌的细胞外囊泡（EV），在局部和远距离细胞间通讯以及清除不需要的物质等多种生物过程中至关重要。近期证据表明，MUC1糖蛋白的过表达促进细胞膜上微囊泡的形成，但这一非平衡过程的潜在机制仍不清楚。本文在Onsager变分原理框架下研究了糖萼驱动的微囊泡形成动力学。利用这一确定性理论，我们首先表明，对于更致密和更厚的糖萼修饰细胞膜，微囊泡形成过程加速，并且可以通过降低离子强度或增强糖萼电离程度来改善。有趣的是，我们进一步发现存在一个最佳的糖萼覆盖率，使得微囊泡形成最快。随后，我们采用随机方法研究了微囊泡形成过程。我们的结果表明，转变速率不是恒定的，从而预测出比确定性方法更短的形成时间。这些发现为糖萼调控膜形态和微囊泡分泌提供了有价值的见解，对理解和调节这些过程具有重要意义。

英文摘要

Microvesicles (MVs), a type of cell-secreted extracellular vesicle (EV), are essential for various biological processes such as local and long-distance intercellular communication and the disposal of unwanted materials. Recent evidence suggests that overexpression of MUC1 glycoproteins facilitates MV formation on the cell membrane, but the mechanism underlying this nonequilibrium process remains unknown. Here, the kinetics of glycocalyx-driven MV formation is studied in the framework of Onsager's variational principle. Using this deterministic theory, we first show that the process of MV formation is accelerated for the denser and thicker glycocalyx-grafted cell membrane and can be improved by reducing the ionic strength or enhancing the degree of glycocalyx ionization. Intriguingly, we further discover that there exists an optimal glycocalyx coverage for the fastest formation of MVs. We then investigated the process of MV formation using a stochastic approach. Our results suggest that the transition rate is not constant, leading to the prediction of a shorter formation time compared to the deterministic approach. These findings provide valuable insights into the regulation of membrane morphology and MV secretion by the glycocalyx, with implications for both understanding and modulating these processes.

发表机构

• University of California San Diego（加州大学圣地亚哥分校）
• Xiamen University（厦门大学）

机构由 AI 辅助整理，请以论文原文为准。