AI 中文总结
本研究揭示果蝇下行神经元中，共享突触前输入的连接在接收神经元内的输入位置更接近，从而建立了网络拓扑与突触空间布局之间的对应关系。
AI 中文摘要
网络拓扑描述神经元之间的连接，而突触布局则规定这些连接在单个细胞内的排列方式。这两种组织水平之间的对应关系尚未被完全理解。在此，我们表明突触前神经元之间的连接性反映在果蝇下行神经元（DNs）内相对输入的位置上。在独立重建的MaleCNS和FlyWire大脑中，跨越数千个单向DN连接，来自同时接触配对DN的输入源，其沿接收者神经突的位置平均比来自其他非DN的输入源更靠近该配对DN的输入，距离分别为9.4和8.0微米。当固定源输入位置并在标准化参考集大小后比较有和没有记录源输入的配对时，同样的排序成立。在BANC和MaleCNS中，共享输入和测量的空间重叠为DN互连提供了互补的预测信息。两个共享一个上升源且相互连接的两个DN，在其识别出的索输出上有所不同。总之，这些发现将三神经元拓扑关系与相对输入位置联系起来，揭示了网络连接性与接收神经元内部空间组织之间的对应关系。
英文摘要
Network topology describes connections between neurons, whereas synaptic placement specifies how those connections are arranged within individual cells. How these levels of organization correspond remains incompletely understood. Here we show that connectivity between presynaptic neurons is reflected in relative input placement within Drosophila descending neurons (DNs). Across thousands of one-way DN connections in the independently reconstructed MaleCNS and FlyWire brains, inputs from sources also contacting the partner DN lay on average 9.4 and 8.0 μm nearer that partner's inputs along the receiver's neurites than other non-DN inputs. The same ordering held when source-input positions were fixed and partners with and without recorded source input were compared after standardizing reference-set size. In BANC and MaleCNS, shared input and measured spatial overlap provided complementary predictive information about DN interconnection. Two interconnected DNs sharing an ascending source differed in their identified cord outputs. Together, these findings link a three-neuron topological relationship to relative input placement, revealing a correspondence between network connectivity and the internal spatial organization of a receiving neuron.
