发表机构
IonLabs（IonLabs）
机构由 AI 辅助整理，请以论文原文为准。AI 中文总结
该研究通过进化智能体比较果蝇连接组与随机化布线的适应度，发现感觉-运动边界的零模型处理显著影响比较结果，且捷径对适应度和嗅觉依赖有重大贡献。
AI 中文摘要
连接组的随机化副本是评估测量到的布线是否重要的常用基线，而答案取决于随机化保留了哪些特征。我们进化了具身觅食智能体，其大脑为压缩的成年果蝇连接组（FlyWire v783；512个细胞类型组和1,000个肯尼恩细胞），同时进化了基于随机化布线的智能体，在预注册实验中使用了十个种子、四种生态和600代。两种标准随机化——列洗牌和保度边交换——将10.6%至10.7%的嗅觉输出直接路由到下行运动组，而连接组中这一比例为0.012%。在注册的主要终点（运行平均适应度）上，未检测到差异；在最后的共同花园探针中，连接组落后于两个对照组（种子均值上分别为-0.22和-0.20适应度单位）。与保留所有感觉输出和运动输入边且仅重连内部的对照组相比，种子均值差异落在±0.10等效界限内（+0.002和-0.074，在同时匹配活动分布的校准下不变），尽管按生态划分，内部列洗牌在四种生态之一、十个种子下领先0.26，但这一领先在十个额外预注册种子中未得到重复。将连接组重连以使其获得捷径，将其适应度提高了0.44（10/10个种子），其对嗅觉的依赖从0.15增加到0.99；分级剂量使两者同步增加；在可比较的交换次数下，全剂量比仅内部的假手术领先0.53（10/10个种子）；而重连相同边界边但不创建捷径的假手术与连接组匹配（+0.007），而全剂量领先其0.60。零模型在感觉-运动边界保留的内容可以决定进化连接组比较的结果，感觉到运动的路径统计应归属于零模型据称保留的度统计之列。
英文摘要
Randomised copies of a connectome are the usual baseline for asking whether measured wiring matters, and the answer depends on what the randomisation preserves. We evolved embodied foraging agents whose brains are a compressed adult Drosophila connectome (FlyWire v783; 512 cell-type groups and 1,000 Kenyon cells) alongside agents built on randomised wiring, in pre-registered experiments with ten seeds, four ecologies and 600 generations. Two standard randomisations, a column shuffle and degree-preserving edge swaps, route 10.6 to 10.7 % of olfactory output directly onto descending motor groups, against 0.012 % in the connectome. On the registered primary endpoint, fitness averaged over the run, no difference was detected; at the last common-garden probe the connectome was behind both controls (-0.22 and -0.20 fitness units on seed means). Against controls that keep every sensory-output and motor-input edge and rewire only the interior, the seed-mean difference lay within a +/-0.10 equivalence bound (+0.002 and -0.074, unchanged under a calibration that also matches activity spread), although per ecology the interior column shuffle was ahead by 0.26 in one of four ecologies at ten seeds, a lead that ten further pre-registered seeds did not replicate. Rewiring the connectome so that it acquires the shortcut raised its fitness by 0.44 (10 of 10 seeds) and its dependence on olfaction from 0.15 to 0.99; graded doses raised both in step; at comparable swap counts the full dose was ahead of an interior-only sham by 0.53 (10 of 10 seeds); and a sham that rewired the same boundary edges without creating shortcuts matched the connectome (+0.007) while the full dose was ahead of it by 0.60. What a null preserves at the sensory-motor boundary can decide an evolutionary connectome comparison, and sensory-to-motor path statistics belong next to the degree statistics a null is said to preserve.
Comments9 pages main text + appendix (27 pages total), 9 figures (3 in the main text). Code, protocols, results and a dated research log: https://doi.org/10.5281/zenodo.22871090 ; https://github.com/gyujeongion/flyconnectome-nulls
