冷大气等离子体对拟南芥种子去污并重塑幼苗真菌微生物群落
Cold atmospheric plasma decontaminates Arabidopsis thaliana seeds and remodels seedling fungal microbiota
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中文总结 AI 辅助
本研究探究冷大气等离子体对拟南芥种子的去污效果及其对幼苗真菌群落的影响，发现CAP能有效减少真菌污染且不影响萌发，并重塑真菌群落组成，为无农药种子处理提供新途径。
中文摘要 AI 辅助
种子相关的微生物影响种子质量、幼苗建立和植物健康，同时也是种子传播病原体的主要来源。冷大气等离子体（CAP）因其抗菌活性，已成为化学种子处理的有前景的替代方案，尽管其对发育中幼苗相关真菌群落的影响仍知之甚少。本研究将来自两个生态型（Columbia和Landsberg erecta）的拟南芥种子暴露于CAP处理5或15分钟。通过麦芽提取物琼脂培养和比浊分析评估种子去污效率，同时通过ITS1扩增子测序表征来自处理和未处理种子的幼苗相关真菌群落。CAP有效减少真菌污染而不影响种子萌发。CAP改变了发育中幼苗相关真菌群落的组成，但这些变化的幅度取决于种子批次和生态型。优势类群在处理后显著下降，而若干低丰度类群的相对丰度增加。这些发现表明，CAP是一种有效的无农药种子去污技术，也能重塑发育中幼苗相关的真菌群落，突显了基于等离子体的种子处理更广泛的生态后果。
英文摘要
Seed-associated microorganisms influence seed quality, seedling establishment, and plant health and also constitute a major source of seed-borne pathogens. Cold atmospheric plasma (CAP) has emerged as a promising alternative to chemical seed treatments because of its antimicrobial activity, although its effects on fungal communities associated with developing seedlings remain poorly understood. Here, Arabidopsis thaliana seeds from two ecotypes (Columbia and Landsberg erecta) were exposed to CAP for 5 or 15 min. Seed decontamination efficiency was assessed by culturing on malt extract agar and nephelometric analyses, while fungal communities associated with seedlings derived from treated and untreated seeds were characterized by ITS1 amplicon sequencing. CAP efficiently reduced fungal contamination without affecting seed germination. CAP altered the composition of fungal communities associated with developing seedlings, but the magnitude of these changes depended on seed batch and ecotype. Dominant taxa markedly declined after treatment, whereas several low-abundance taxa increased in relative abundance. These findings demonstrate that CAP is an effective pesticide-free technology for seed decontamination and can also reshape fungal communities associated with developing seedlings, highlighting broader ecological consequences of plasma-based seed treatments.
发表机构
- Sorbonne Université（索邦大学）
- CNRS（法国国家科学研究中心）
- Inserm（法国国家健康与医学研究院）
- Muséum National d’Histoire Naturelle（法国国家自然历史博物馆）
- École Polytechnique（巴黎综合理工学院）
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