雄性果蝇中枢神经系统连接组的预注册谱与认证混合分析
Pre-registered spectral and certified mixing analysis of the male Drosophila central nervous system connectome
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中文总结 AI 辅助
该研究预注册测试了雄性果蝇中枢神经系统连接组随机游走的五个假设，发现谱间隙远小于预测且混合深度不可认证，慢模态源于重建边缘的几乎闭合神经元集，提示需显式处理这些边界集。
中文摘要 AI 辅助
我们报告了一项预注册测试，针对雄性果蝇中枢神经系统连接组（166,700个神经元，1.24亿个突触）上的突触流随机游走，测试了五个假设。在该随机游走中，游走者按突触数量比例移动到突触后伙伴。这些假设涉及谱间隙及其对颈部连接束的依赖性、输入归一化符号映射的前导模态的局域化、由Dobrushin系数测量的认证混合深度、其对每个突触检测置信度的鲁棒性，以及符号谱对神经递质符号约定的依赖性。在公开注册和代码冻结后，每个假设被测试一次。两个假设成立：主导符号谱的两个神经元光感受器模态是极小输入归一化的伪影，通过中位数输入下限消除，且它们不依赖于符号约定。三个假设失败：在每连接五个突触的阈值下，谱间隙比预测小一个数量级（|λ2| = 0.9965），且链在128步后经认证远未混合，并且在任何测试的置信度水平下，混合深度均未得到认证鲁棒性。探索性分析将慢模态追溯到脊髓的11个神经元，其中7个为中胸传出神经元，在弱连接被丢弃后这些神经元几乎闭合；在FlyWire雌性脑的层板中发现类似的边界集；显示保留细胞类型布线的零模型重现了慢模态；并显示由置信度数据构建的精确链在预注册置信度水平下，32步后的深度剖面变化最多为0.017，远小于最坏情况证书允许的范围。连接组的随机游走摘要由重建边缘处的几乎闭合集主导，除非这些集被显式处理。
英文摘要
We report a pre-registered test of five hypotheses about the synapse-flow random walk on the connectome of the male Drosophila central nervous system (166,700 neurons, 124 million synapses), in which a walker moves to a postsynaptic partner in proportion to synapse number. The hypotheses concern the spectral gap and its dependence on the neck connective, the localisation of the leading modes of an input-normalised signed map, the certified mixing depth measured by the Dobrushin coefficient, its robustness to per-synapse detection confidence, and the dependence of the signed spectrum on the neurotransmitter sign convention. After public registration and a code freeze, each was tested once. Two held: the two-neuron photoreceptor modes that lead the signed spectrum are an artefact of normalising by very small inputs, removed by an input floor at the median, and they do not depend on the sign convention. Three failed: at a threshold of five synapses per connection the spectral gap is an order of magnitude smaller than predicted ($|λ_2| = 0.9965$) and the chain is certifiably far from mixed after 128 steps, and at no confidence level tested was the mixing depth certifiably robust. Exploratory analyses trace the slow mode to eleven neurons of the cord, seven of them mesothoracic efferent neurons, that become nearly closed once weak connections are dropped, find a similar boundary set in the lamina of the FlyWire female brain, show that null models preserving cell-type wiring reproduce the slow modes, and show that exact chains built from the confidence data change the depth profile after 32 steps by at most 0.017 at the pre-registered confidence level, far less than the worst-case certificate allows. Random-walk summaries of connectomes are dominated by nearly closed sets at the edges of the reconstruction unless these are treated explicitly.
发表机构
- Tel Aviv University（特拉维夫大学）
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