从OpenSPIM到FlowSPIM：通过迭代设计过程增强光片显微镜的多功能性
From OpenSPIM to FlowSPIM: Enhancing the versatility of a light sheet microscope through an iterative design process
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中文总结 AI 辅助
本文通过迭代设计过程，从OpenSPIM平台逐步构建模块化光片显微镜FlowSPIM，增强其多功能性，并引入基于流动的孵育技术以适配不同样本和实验条件。
中文摘要 AI 辅助
光片显微镜非常适合大型生物样本的三维成像，然而关于将显微镜设计适配到不同实验需求的系统性文献仍然有限。我们展示了用于设计和构建模块化光片显微镜并将其适配到不同样本类型和实验条件的迭代过程。从一个精简的OpenSPIM平台出发，我们通过实施新的硬件（如额外的激光通道和光学滤光片）以及新的样本处理协议，逐步使我们的显微镜更具多功能性。在最终迭代中，FlowSPIM是一种用于三维组织培养的新型基于流动的孵育技术。
英文摘要
Light sheet microscopy is ideal for the 3D imaging of large biological specimens, yet systematic documentation on adapting microscope designs to diverse experimental requirements remains limited. We present the iterative process used to design and build a modular light-sheet microscope and adapt it to different sample types and experimental conditions. Starting from a stripped-down OpenSPIM platform, we gradually make our microscope more versatile by implementing new hardware, such as additional laser channels and optical filters, and new protocols for sample handling. In its final iteration, FlowSPIM is a new flow-based incubation technique for 3D tissue culture.
发表机构
- University of Oslo（奥斯陆大学）
机构由 AI 辅助整理，请以论文原文为准。
