发表机构
Center for Biological Science and Technology, Advanced Institute of Natural Sciences, Beijing Normal University（北京师范大学自然科学高等研究院生物科学与技术中心）
机构由 AI 辅助整理，请以论文原文为准。AI 中文总结
ChatT2提出一种基于大语言模型的多智能体框架，通过导师、执行者和评估者协作，结合思维链和检索增强生成，解决微生物天然产物研究中新手面临的复杂挑战，提供专家和新手均可用的探索工具。
AI 中文摘要
对微生物天然产物（NPs）的科学探究给新手带来了重大挑战，这主要源于微生物系统的复杂性、生化多样性、技术技能要求，以及生物信息学和数据分析过程的严苛需求。为解决这些问题，我们引入了ChatT2，一个基于大语言模型（LLM）的智能体，它专门针对细菌II型聚酮化合物的独特特征而定制。这些聚酮化合物构成了一个结构独特且在治疗上具有重要意义的NP家族。ChatT2是在一个自主多智能体框架内开发的，该框架由导师（mentor）、执行者（executor）和评估者（evaluator）组成，各自承担明确的职责。导师充当ChatT2与用户之间的中介，利用思维链提示来细化用户的意图。在导师的指导下，执行者通过检索增强生成技术综合多模态信息，并无缝集成生物信息学和化学信息学工具。评估者最终对执行者的输出进行评估，以确保检索信息的丰富性和准确性。我们的研究突显了ChatT2如何通过这种多智能体框架设计，应对通用大语言模型在理解有限、专业语料库和复杂生物信息方面所面临的挑战，并为专家和新手提供了一种探索各种感兴趣的NPs的有价值工具。ChatT2网络服务器可通过此https URL访问。
英文摘要
Scientific investigations into microbial natural products (NPs) present significant challenges for novices, largely due to the complexity of microbial systems, biochemical diversity, technical skill requirements, and the demands of bioinformatics and data analysis processes. To address these issues, we introduce ChatT2, a large language model (LLM)-based agent that is specifically tailored to the unique characteristics of bacterial type II polyketides. These polyketides form a structurally distinct and therapeutically important NP family. ChatT2 was developed within an autonomous multiagent framework composed of a mentor, an executor, and an evaluator, each with defined responsibilities. The mentor acts as an intermediary between ChatT2 and the user, utilizing chain-of-thought prompting to refine the intent of the user. Under the guidance of the mentor, the executor synthesizes multimodal information via retrieval-augmented generation techniques and seamlessly integrates bioinformatics and cheminformatics tools. The evaluator ultimately assesses the output of the executor to ensure the richness and accuracy of the retrieved information. Our research highlights how ChatT2, designed with this multiagent framework, addresses the challenges faced by general LLMs in terms of understanding limited, specialized corpora and complex biological information and provides both experts and novices with a valuable tool for exploring various NPs of interest. The ChatT2 webserver can be accessed at https://chatt2.site/#/chat.
