发表机构
Sun Nuclear Corporation; University of Michigan College of Dentistry; University of Iowa; University of Illinois College of Dentistry（太阳核公司; 密歇根大学牙学院; 爱荷华大学; 伊利诺伊大学牙学院）
机构由 AI 辅助整理，请以论文原文为准。AI 中文总结
本研究提出基于定量超声的衰减系数斜率与背向散射强度作为牙周炎症诊断生物标志物，通过猪模型验证其可行性，发现ACS显著区分炎症，有望补充现有临床标准。
AI 中文摘要
在美国，30岁及以上的成年人中，每10人就有4人受到牙周病的影响，牙周病涵盖了一系列炎症性疾病。目前，临床上采用一种主观、侵入性且半定量的方法，称为探诊出血，用于炎症评估。本研究的长期目标是通过提出基于定量超声（QUS）的生物标志物用于炎症诊断和监测，填补牙科当前临床诊断的空白。作为该领域的早期工作之一，我们通过一项纵向临床前猪模型研究，探究了两个QUS参数用于表征牙龈组织牙周炎症：衰减系数斜率（ACS）和背向散射强度（BSI）。我们的研究包括八头猪，在从第0周（健康）到第6周（炎症接种后）的四个双周时间点，对其所有象限的第三前磨牙（PM3-Mes）邻面进行口内成像（24 MHz）。此外，我们比较了健康状态下第二磨牙（M2-Dis）舌侧/腭侧与颊侧周围牙龈组织。结果显示，使用混合效应分析，所有炎症时间点的牙龈ACS均显著低于健康牙龈ACS（1.69 ± 0.53 dB/cm.MHz）（第6周：1.08 ± 0.25 dB/cm.MHz）。BSI的纵向比较未显示任何统计学显著性。M2-Dis舌侧/腭侧与颊侧的牙龈ACS和BSI未表现出任何统计学显著性。这些ACS呈线性相关，R平方为0.89，相关斜率为0.87。对于BSI，Bland-Altman分析显示平均BSI无差异，尽管变异性较高。这些发现凸显了超声成像结合QUS在补充当前牙科标准护理方面的巨大潜力。
英文摘要
Four out of 10 adults aged 30 years or older in the USA are impacted by periodontal diseases which span a spectrum of inflammatory conditions. Currently, a subjective, invasive and a semi-quantitative approach, termed bleeding on probing, is employed in clinics for inflammation assessment. The long-term goal of this study is to fill the current clinical diagnostic gap in dentistry by proposing quantitative ultrasound (QUS)-based biomarkers for inflammation diagnosis and monitoring. Here, as one of the early works in this area, we investigated two QUS parameters for characterizing periodontal inflammation in gingival tissues using a longitudinal preclinical porcine study: attenuation coefficient slope (ACS) and backscatter intensity (BSI). Our study included eight pigs imaged intraorally (24 MHz) at four bi-weekly timepoints from week 0 (healthy) to week 6 (post inflammation inoculation) at their interproximal sites of the third premolars (PM3-Mes) from all quadrants. Moreover, we compared gingival tissues surrounding a tooth at lingual/palatal side versus buccal side at the second molar (M2-Dis) in healthy condition. Our results showed that gingival ACS at all inflammation timepoints were significantly lower than healthy gingival ACS (1.69 \pm 0.53 dB/cm.MHz) using a mixed effect analysis (week 6: 1.08 \pm 0.25 dB/cm.MHz). Longitudinal comparison of BSI did not demonstrate any statistical significance. Gingival ACS and BSI at lingual/palatal versus buccal sides of M2-Dis did not exhibit any statistical significance. These ACSs were linearly correlated with R-squared = 0.89 and a correlation slope of 0.87. For BSI, Bland-Altman analysis demonstrated no difference in mean BSI, although variability was high. These findings highlight the promising potential of ultrasonography paired with QUS to complement current standard of care in dentistry.
