发表机构
University of Bath; Istituto Italiano di Tecnologia; University of Southampton（巴斯大学; 意大利理工学院; 南安普顿大学）
机构由 AI 辅助整理，请以论文原文为准。AI 中文总结
本文提出光学微电极阵列，通过差分读出分离电信号与机械信号，实现心肌细胞电兴奋和收缩的无标记同步检测，支持心脏毒性高通量筛选。
AI 中文摘要
同时评估电兴奋和机械收缩对于理解心肌细胞功能至关重要，然而这两个过程通常通过分离的技术或有创方式进行测量。在此，细胞电活动的变化调节光学微电极中的局部电荷重新分布，并转化为荧光信号，而细胞收缩引起膜位移，为光学读出贡献额外的机械分量。通过比较搏动细胞中获得的记录与抑制收缩后获得的记录，我们将动作电位相关的静电转导与收缩驱动的膜运动分离开来。该方法提供了一条无标记途径，以支持用于心脏毒性筛选和机电传感的高通量体外检测。同时，它揭示了在心肌细胞生物电子学和力学中部署的基于膜的光学器件的物理机制。
英文摘要
Simultaneous assessment of electrical excitation and mechanical contraction is essential for understanding cardiac cell function, yet these two processes are commonly measured with separate techniques or invasively. Here, changes in cellular electrical activity modulate local charge redistribution in optical microelectrodes and are converted into fluorescence signals, while cell contraction induces membrane displacement that contributes an additional mechanical component to the optical readout. By comparing recordings obtained in beating cells with those acquired after inhibition of contraction, we separate action-potential-associated electrostatic transduction from contractility-driven membrane motion. The approach offers a label-free route to support high-throughput in vitro assays for cardiotoxicity screening and electromechanical sensing. Concurrently, it unfolds the physical mechanisms governing membrane-based optical devices deployed in cardiac cell bioelectronics and mechanics.
Comments16 pages, 4 main figures, 6 supplementary items
