发表机构
University of Oulu; VTT, Technical Research Centre of Finland（奥卢大学; 芬兰技术研究中心）
机构由 AI 辅助整理，请以论文原文为准。AI 中文总结
本文提出利用微控制器结合拉曼光谱反馈自动调节电压，去除等离激元纳米孔中的金纳米粒子聚集体，以实现DNA和蛋白质的自动化高通量单分子SERS检测。
AI 中文摘要
等离激元纳米孔已被用于利用电场偏压捕获单个纳米粒子，以实现单分子表面增强拉曼光谱（SM-SERS）检测和测序。尽管它们表现出高灵敏度，但去除纳米孔中的纳米粒子聚集体非常费力，这阻碍了生物传感应用。在此，我们的方法是使用微控制器改变电压，以通过拉曼光谱反馈去除纳米孔中的纳米粒子聚集体。吸附在纳米粒子表面的分子的SM-SERS光谱可以在足够长的时间内获取，从而使微控制器能够在不到100毫秒的时间内分析它们，并触发电压变化以去除纳米粒子聚集体。我们的系统有望实现DNA和蛋白质的自动化和高通量SM-SERS检测与分析。
英文摘要
Plasmonic nanopores have been used to trap single nanoparticles using electric bias for single-molecule surface enhanced Raman spectroscopic (SM-SERS) detection and sequencing. While they exhibit high sensitivity, removal of nanoparticle clusters in the nanopore is so labor intensive that it hampers biosensing applications. Here, our approach is to use a microcontroller to change the electric voltage to remove the nanoparticle clusters in the nanopore with feedback by the Raman spectra. The SM-SERS spectra of the molecules adsorbed on nanoparticle surface can be acquired over a sufficiently long period, so that the microcontroller can analyze them with <100 ms and trigger the voltage change to remove the nanoparticle cluster. Our system is promising for automatic and high-throughput SM-SERS detection and analysis of DNA and proteins.
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