发表机构
Purdue University; KU Leuven; Maastricht University（普渡大学; 荷语鲁汶大学; 马斯特里赫特大学）
机构由 AI 辅助整理，请以论文原文为准。AI 中文总结
本研究通过实验成像与计算建模，揭示鸡胚卵黄囊中器官尺度间质流模式，其由渗漏与引流空间分离驱动，对生物运输具有重要意义。
AI 中文摘要
间质流在药物递送、血管生成、癌症和水肿中发挥关键作用。近期研究表明，间质可以提供能够支持长距离物质运输的连接通路。然而，生理性间质流的空间尺度仍不清楚：尽管存在连接通路，流动可能由附近的血管滤过主导，或延伸至更长的距离。在此，我们在鸡胚卵黄囊中识别出器官尺度的间质流模式，并阐明了决定其空间尺度的机制。卵黄囊作为一个器官尺度的间质区域，使得无需截断即可识别大规模流动模式。我们的方法将实验成像与整个卵黄囊中血液和间质流耦合的计算建模相结合。尺度分析确定了一个水力传导率比值，该比值控制从小尺度到器官尺度流动的转变。在器官尺度模式中，我们发现间质流速度显著大于经血管流动速度。从机制上讲，器官尺度模式源于血管对间质液的渗漏和引流的空间分离。这些发现对间质流介导的生物运输具有重要意义，表明流动介导的信号可能被局部感知，但由非局部产生。
英文摘要
Interstitial flow plays a key role in drug delivery, angiogenesis, cancer, and edema. Recent studies have identified connected interstitial pathways that can transport material over long distances. However, the spatial scale of physiological interstitial flow remains unclear: despite the existence of connected pathways, flow may be dominated by nearby vascular filtration or extend over longer distances. Here, we identify organ-scale interstitial flow patterns in the chick yolk sac and elucidate the mechanisms that determine their spatial scale. The yolk sac contains an organ-scale interstitial region that enables large-scale flow patterns to be identified without truncation. Our approach combines experimental imaging with computational modeling of coupled blood and interstitial flow in the whole yolk sac. Scaling analysis identifies a hydraulic conductivity ratio that controls the transition from small-scale to organ-scale flow. In organ-scale patterns, we find interstitial flow speed to be substantially greater than the transvascular flow speed. Mechanistically, the organ-scale patterns arise from the spatial segregation of the leakage and drainage of interstitial fluid from the vessels. These findings have important implications for biological transport by interstitial flow, suggesting that flow-mediated cues may be sensed locally but generated nonlocally.
Comments28 pages, 10 figures, 1 table; includes Supplementary Materials; revised figure captions
