脉冲偶极EPR实验中分子内与分子间贡献的直接分离
Direct separation of intra- and inter-molecular contributions in pulse dipolar EPR experiments
- Department of Chemistry and Applied Biosciences（化学与应用生物系）
机构由 AI 辅助整理，请以论文原文为准。
AI总结：
本文提出一种无需拟合、直接分离脉冲EPR偶极光谱中分子内与分子间贡献的新方法，适用于自旋浓缩样品及液-液相分离研究。
AI中文摘要：
我们提出了一种直接分离脉冲EPR偶极光谱（PDS）实验中分子内（形状因子）和分子间（背景）贡献的方法。通过重新排列在两个不同双自由基浓度下测得的数据来实现形状因子/背景分离，从根本上无需任何拟合。这种新的数据处理方法使得我们能够分析自旋浓缩样品的PDS数据，而此前在这些样品中进行背景分离是不现实的。特别是，所提出的方法对于自旋标记生物分子的研究具有重要意义，尤其是在液-液相分离（LLPS）方面。此外，我们提出了一种简单的测量和数据处理方案，以分离LLPS样品中各相的PDS贡献。文中还讨论了RIDME实验中形状因子/背景分离的方案。
英文摘要：
We present a direct method of separating intra- (form factor) and inter-molecular (background) contri- butions in pulse EPR dipolar spectroscopy (PDS) experiments. The form factor/background separation is accomplished by rearranging data measured at two different biradical concentrations, without any fitting fundamentally required. This new data processing method allows to analyse PDS data from spin concen- trated samples for which background separation was previously unrealistic. In particular, the presented method would be of high importance for studies of spin labeled biomolecules, especially in respect to the liquid-liquid phase separation (LLPS). Furthermore, we propose a simple measurement and data processing protocol to separate the PDS contributions from each phase in LLPS samples. The protocol for the form factor/background separation for RIDME experiment is also discussed
