发表机构
Pontifical Catholic University of Rio de Janeiro; Centro Nacional de Ressonância Magnética Nuclear, Departamento de Bioquímica Médica, ICB/CCS/UFRJ; Instituto de Física, Universidade Federal Fluminense（里约热内卢天主教大学; 里约热内卢联邦大学生物医学化学系国家核磁共振中心; 弗鲁米嫩塞联邦大学物理研究所）
机构由 AI 辅助整理，请以论文原文为准。AI 中文总结
本研究利用暗光阱在排斥区稳定捕获活酵母细胞数小时，避免光损伤，实现长时间观察细胞繁殖。
AI 中文摘要
光镊是细胞学领域的一种多功能工具，能够实现对单个细胞的捕获和操控。然而，即使以低光功率和短时间操作，激光照射也会对生物物质造成光损伤。在此，我们展示了利用工作在光-物质相互作用排斥区的暗光阱，在体外对单个活体酿酒酵母细胞进行稳定捕获。与标准光镊相比，我们的暗光阱可将细胞捕获数小时，且对其形态和繁殖周期的干扰可忽略不计，这为对活生物体进行长时间实验开辟了新的可能性，例如在激光捕获下观察细胞繁殖。
英文摘要
Optical tweezers are a versatile tool in the domain of cytology, enabling trapping and manipulation of individual cells. However, the incidence of laser light causes photodamage to biological matter, even when operating at low optical powers and over short periods of time. Here, we demonstrate stable trapping of single living Saccharomyces cerevisiae yeast cells in vitro using a dark optical trap operating in the repulsive regime of light-matter interactions. In contrast to standard tweezers, our dark optical trap confines cells for hours with negligible disruption to their morphology and reproduction cycle, opening up new possibilities for long duration experiments with living organisms such as the observation of cell reproduction under laser trapping.
Comments8 pages, 7 figures, 1 table. Supplemental Material (3 movies) submitted separately
