酶传播与生物聚合物迁移率的同时测量阐明了致密生物聚合物基质的定向降解
Simultaneous measurements of enzyme propagation and biopolymer mobility elucidate the directional degradation of a dense biopolymer matrix
浏览论文内容
中文总结 AI 辅助
该研究通过同时追踪酶扩散与生物聚合物降解，明确了致密生物聚合物基质降解与酶传播的关系，建立了优化相关酶过程的定量框架。
中文摘要 AI 辅助
生物聚合物的酶降解是生物技术应用及自然过程（如食物消化、癌症发展）的关键基础，但致密基质中酶传播与底物降解的相互作用仍未明确，尤其不清楚致密基质的降解是促进还是阻碍酶传播。我们在模型系统中以时空分辨率同时追踪单向酶扩散与生物聚合物降解，发现酶扩散与催化活性解耦，而降解前沿的推进由酶扩散、反应动力学及酶缓慢失活决定。这些发现建立了定量框架，可优化生物医学、生物质增值及纳米技术领域的酶过程。
英文摘要
Enzymatic degradation of biopolymers underpins critical processes in biotechnological applications and natural processes, such as food digestion and cancer development, yet the interplay between enzyme propagation in a dense substrate and substrate degradation remains unresolved. It remains especially unclear whether degradation of a dense matrix facilitates or impedes enzyme propagation. We simultaneously track with spatiotemporal resolution unidirectional enzyme diffusion and biopolymer degradation in a model system. We demonstrate that enzyme diffusion is decoupled from catalytic activity, while the degradation front progression is dictated by enzyme diffusion, reaction kinetics and slow enzyme deactivation. These findings establish a quantitative framework to optimize enzymatic processes for applications in biomedicine, biomass valorization, and nanotechnology.
发表机构
- Laboratoire Charles Coulomb (L2C), CNRS, Université de Montpellier（蒙彼利埃大学）
- TBI, Université de Toulouse, CNRS, INRAE, INSA（图卢兹大学）
- IATE, INRAE, Institut SupAgro, Université de Montpellier（蒙彼利埃大学）
- IBMM, Université de Montpellier, CNRS, ENSCM（蒙彼利埃大学）
- Institut Universitaire de France（法兰西学院）
机构由 AI 辅助整理，请以论文原文为准。
