发表机构
Forschungszentrum Juelich; University of Wuppertal; University of Twente; Netherlands Institute for Neuroscience, Royal Netherlands Academy of Arts and Sciences（于利希研究中心; 伍珀塔尔大学; 特文特大学; 荷兰神经科学研究所，荷兰皇家艺术与科学院）
机构由 AI 辅助整理，请以论文原文为准。AI 中文总结
该研究针对脑成像中纤维交叉分辨难题，提出基于瑞利-甘斯散射理论近似的三维散射光成像方法，可从一维SLI测量提取神经纤维三维取向，经体模验证后应用于脑组织并与3D-PLI对比。
AI 中文摘要
在微观尺度上理解大脑的三维纤维结构，对于揭示其结构连接性与功能至关重要。基于偏振的光学成像技术（3D-PLI）可实现对单根神经纤维取向的高保真重建，但难以分辨纤维交叉，而纤维交叉是恢复完整连接组的关键。基于散射的成像技术可获取三维取向纤维的结构因子，通过在多个方位角照明角度下探测固定散射角，可利用计算散射光成像（SLI）恢复平面内纤维取向与交叉。然而，尽管包含三维信息，此前一直缺乏提取完整三维取向的理论框架。本文引入瑞利-甘斯散射理论的简单类比近似，以从一维SLI测量中提取神经纤维的三维取向；该理论通过由双光子光刻技术制备的、包含2微米厚杆状晶格的倾斜显微玻璃体模得到验证；最后将该方法应用于脑组织样本，并与3D-PLI进行对比。
英文摘要
Understanding the 3D fiber architecture of the brain at the microscopic scale is essential for revealing its structural connectivity and function. Polarization-based optical imaging (3D-PLI) techniques enable high-fidelity reconstruction of single nerve fiber orientations but struggle to resolve fiber crossings, which are critical for recovering the full connectome. Scattering-based imaging provides access to the structure factor of three-dimensionally oriented fibers. By probing a fixed scattering angle under multiple azimuthal illumination angles, in-plane fiber orientations and crossings can be recovered using computational scattered light imaging (SLI). However, despite containing 3D information, a theoretical framework to extract full 3D orientations has been lacking. In this talk, a simple analogical approximation of Rayleigh-Gans scattering theory is introduced to extract the 3D orientation of nerve fibers from one-dimensional SLI measurements. The theory is validated using tilted microscopic glass phantoms consisting of 2 um-thick rod lattices fabricated by two-photon lithography. Finally, the method is applied to brain tissue samples and compared with 3D-PLI.
Comments24 pages, 5 figures, conference: https://spie.org/optics-photonics/presentation/3D-scattered-light-imaging--extracting-3D-fiber-orientations-from/14209-12
