色散调控的微纳盖玻片用于多模态合成成像

中文摘要 AI 辅助

多模态光学成像可实现样品的全面表征，但传统上受限于复杂且庞大的仪器设备。本文提出一种色散调控的微纳盖玻片，可在标准显微镜架构内无缝集成明场、微分、荧光和全息成像模态，无需硬件改造或重新校准。该微纳盖玻片利用可扩展的亚波长多层膜调控时空色散，以定制高维传递函数。作为示例，通过简单调节照明波长即可在四种成像模态间灵活切换。最后证实，多模态图像合成可提供空间配准的结构与分子信息，用于更全面的生物分析。该方法为高级成像提供了易用、可扩展且灵活的解决方案，可扩展至传感和计算领域的其他多路复用光学系统。

英文摘要

Multimodal optical imaging provides comprehensive sample characterization but has traditionally been hindered by complex and bulky instrumentation. Here, we propose a dispersion-engineered meta-coverslip to seamlessly integrate bright-field, differential, fluorescence, and holographic imaging modalities within a standard microscope architecture, requiring no hardware modification or realignment. The meta-coverslip utilizes a scalable subwavelength multilayer film to engineer spatio-temporal dispersion for a customized high-dimensional transfer function. As an example, we demonstrate flexible switching among the four imaging modalities by simply tuning the illumination wavelengths. Lastly, we demonstrate that the synthesis of multimodal images can provide spatially registered structural and molecular information for more comprehensive biological analysis. Our approach provides an accessible, scalable, and flexible solution for advanced imaging and is extensible to other multiplexed optical systems in sensing and computing.

发表机构

• Tsinghua University（清华大学）
• Nanyang Technological University（南洋理工大学）

机构由 AI 辅助整理，请以论文原文为准。