纳米多孔铜薄膜作为紫外表面增强拉曼散射（UV-SERS）平台：灵敏度与手性识别能力
Nanoporous Copper Films as Platform for UV-SERS: Sensitivity and Ability to Perform Chiral Discrimination
- Zhejiang University of Technology（浙江工业大学）
- Istituto Italiano di Tecnologia（意大利理工大学）
- Zhejiang University（浙江大学）
- Università degli Studi di Modena e Reggio Emilia（摩德纳和雷焦艾米利亚大学）
- Umeå University（于默奥大学）
- University of Brescia（布雷西亚大学）
- China Jiliang University（中国计量大学）
机构由 AI 辅助整理，请以论文原文为准。
AI总结：
本文制备干法合成的纳米多孔Cu薄膜作为UV-SERS衬底，其可低至10 μM检测腺嘌呤，能无手性选择剂区分L/D色氨酸，为UV-SERS生物传感和手性分析提供新平台。
AI中文摘要：
紫外（UV）区域的表面增强拉曼散射（SERS）为生物分子检测提供了重要优势，包括共振增强和减少荧光干扰。然而，开发兼具低成本、可重复性和化学稳定性的UV-SERS衬底仍具挑战性。本文采用干法合成方法在硅衬底上制备纳米多孔铜（Cu）和氧化铜（CuO）薄膜，并以腺嘌呤为拉曼报告分子，在325 nm激发下系统评估其UV-SERS性能。在所研究的衬底中，纳米多孔Cu表现出最强的增强效果，可实现腺嘌呤的低至10 μM检测。相比之下，在532 nm激发下未观察到可检测的腺嘌呤拉曼信号，表明该增强由UV诱导的化学电荷转移机制主导，而非传统的电磁增强。Cu衬底还可对链霉亲和素（作为测试蛋白）进行UV拉曼光谱分析，更有趣的是，无需手性选择剂或额外表面功能化，仅通过UV-SERS强度差异即可区分L-色氨酸和D-色氨酸。通过改变金属蒸发过程中的衬底旋转速度，可调节对映选择性响应，得到L型相对于D型的强度比为1.10至2.35，证明衬底形貌在手性识别中的关键作用。干法合成的纳米多孔薄膜提供了更简单、可扩展且无配体的制备策略，同时具备对映选择性检测的额外能力。这些发现确立了干法制备的纳米多孔Cu薄膜作为UV-SERS生物传感和无标记手性分析的有前景平台。
英文摘要：
Surface enhanced Raman spectroscopy (SERS) in the ultraviolet (UV) region offers important advantages for biomolecular detection, including resonance enhancement and reduced fluorescence interference. However, the development of UV SERS substrates that combine low cost, reproducibility, and chemical stability remains challenging. Here, we employ a dry synthesis approach to fabricate nanoporous Cu and copper oxide (CuO) films on silicon substrates and systematically evaluate their UV SERS performance using adenine as a Raman reporter under 325 nm excitation. Among the substrates investigated, nanoporous Cu exhibits the strongest enhancement, enabling adenine detection down to 10 microM. In contrast, no detectable adenine Raman signal is observed under 532 nm excitation, indicating that the enhancement is dominated by a UV induced chemical, charge transfer mechanism rather than conventional electromagnetic enhancement. The Cu substrates further enable the UV Raman spectroscopy of streptavidin, as a test protein, and, more interestingly, the discrimination between L and D tryptophane based solely on differences in UV SERS intensity, without chiral selectors or additional surface functionalization. By varying the substrate rotation speed during metal evaporation, the enantioselective response can be tuned, yielding L over D intensity ratios from 1.10 to 2.35 and demonstrating the critical role of substrate morphology in chiral discrimination. The dry synthesized nanoporous films provide a simpler, scalable, and ligand free fabrication strategy while offering additional capability for enantioselective detection. These findings establish dry processed nanoporous Cu films as promising platforms for UV-SERS biosensing and label free chiral analysis.
