呈现三维纤维素微纤丝结构的木材双折射彩色光学轮廓分析
Birefringent-colored optical profiling of wood presenting the 3D cellulose microfibril architecture
- National Yang Ming Chiao Tung University（国立阳明交通大学）
机构由 AI 辅助整理，请以论文原文为准。
中文总结 AI 辅助
本研究建立光学模型模拟木材双折射彩色显微图像，通过偏光显微镜结合延迟波片实现三维纤维素微纤丝结构的轮廓分析，三种识别方法结果一致，该高通量方法可为相关领域提供自动化分析框架。
中文摘要 AI 辅助
偏光显微镜下的木材切片因微纤丝中纤维素的双折射特性，会呈现出青色、蓝色、品红色、黄色及蓝灰色等一系列干涉色。本研究建立了一个光学模型来模拟木材的双折射彩色显微图像，考虑了线、螺旋、环、交叉螺旋、扭曲螺旋这五种典型的细胞壁结构。利用延迟波片区分不同取向的微纤丝，每种结构会呈现出独特的双折射彩色纹理，通过这些鲜艳的颜色可展现其潜在的三维结构。研究对巨桉（Eucalyptus grandis）的树干和细枝横截面进行了双折射彩色轮廓分析，比较了三种识别方法：可见微纤丝趋势、双折射彩色光学纹理、模拟查找库，三种方法得到的结果一致，证明双折射彩色标记高效且准确。电子显微镜和原子力显微镜无法分辨嵌入木质素和半纤维素基质中的纤维素微纤丝，而偏光显微镜通过选择性检测双折射纤维素克服了这一问题，能够准确识别复杂的螺旋结构。该方法可对复杂生物材料组成进行统计和空间分析，这种高通量光学方法能同时分析数十个细胞，为植物科学、生物力学及仿生纤维素材料领域提供了潜在的全自动化分析框架。
英文摘要
Wood slices under a polarized optical microscope show a spectrum of interference colors, such as cyan, blue, magenta, yellow, and bluish gray because of the birefringent cellulose in the microfibrils. An optical model is established to simulate the birefringent-colored micrograph of wood. Five typical cell wall architectures, line, helix, ring, crossed helix, and twisted helix, are considered. With a retardation wave plate to distinguish fibrils of different orientations, each structure displays its unique birefringent-colored texture, presenting its underlying 3D structure with the vivid colors. Cross sections of the trunk and twig of Eucalyptus grandis presented birefringent-colored profiles. Three identification methods were compared: visible fibril trends, birefringent-colored optical textures, and the simulated look-up library. The three methods gave consistent results, proving that the birefringent-color tags are efficient and accurate. Electron and atomic force microscopy are unable to resolve cellulose microfibrils embedded in the lignin and hemicellulose matrix. Polarized optical microscopy overcomes this by selectively detecting birefringent cellulose, enabling accurate identification of complex helical structures. This method enables statistical and spatial analysis of complex biomaterial compositions. Capable of profiling dozens of cells simultaneously, this high-throughput optical method provides a potentially fully automated analysis framework for plant science, biomechanics, and bioinspired cellulose materials.
