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采用校准虚拟锥束针孔几何结构的全场荧光计算机断层扫描(F3CT)

Full-field fluorescence computed tomography (F3CT) using a calibrated virtual cone-beam pinhole geometry

Thomas Zillhardt, Yunhui Chen, Alexander Rack, Matthew Veale, Matt Wilson, Philip J. Withers, Nicola Viganò

arXiv 2608.28275首次发表:更新:

AI 中文总结

该研究提出基于同步辐射的F3CT技术,结合针孔光阑与能量分辨二维探测器,经两阶段校准-重建工作流程实现三维元素Mapping,可用于生物、地质样本成像,为三维X射线荧光成像及关联断层扫描提供新方案。

AI 中文摘要

我们提出了F3CT,这是一种基于同步辐射的高光谱全场荧光计算机断层扫描技术,它结合针孔光阑与能量分辨二维探测器,避免了光栅扫描。虚拟锥束模型与两阶段校准-重建工作流程可在全场照明下实现三维元素Mapping。该方法在生物与地质样本上得到验证,解析了斑马鱼组织中的银染色分布及岩芯中的高能荧光信号。在相同实验几何下依次获取的相衬断层图提供了配准的结构背景。F3CT为三维X射线荧光成像提供了高通量路径,为结构与化学关联断层扫描奠定基础,具备未来原位与 operando 实施的潜力。

英文摘要

We present F3CT, a synchrotron-based hyperspectral full-field fluorescence computed tomography technique that avoids raster scanning by combining a pinhole aperture with an energy-resolving 2D detector. A virtual cone-beam model and two-stage calibration-reconstruction workflow enable 3D elemental mapping under full-field illumination. The method is demonstrated on biological and geological specimens, resolving silver-stain distributions in zebrafish tissue and high-energy fluorescence signatures in rock cores. Phase-contrast tomograms acquired sequentially under the same experimental geometry provide co-registered structural context. F3CT provides a high-throughput route to 3D XRF imaging and establishes a foundation for correlated structural and chemical tomography, with potential for future in-situ and operando implementations.

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