用于体内体积动态成像的超快光学相干弹性成像技术
Ultrafast optical coherence elastography for volumetric and dynamic in vivo imaging
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中文总结 AI 辅助
本研究提出超快光学相干弹性成像（ultrafast OCE）框架，结合同步多相位采集与抗混叠解调策略，实现高帧率、抗运动伪影的体内体积动态生物力学成像，为相关领域提供新手段。
中文摘要 AI 辅助
在体高时空分辨率成像生物组织的力学特性，对理解生理功能和疾病进展至关重要。光学相干弹性成像（OCE）可实现无标记、微米级的组织生物力学特性 mapping，但受限于采集速度慢和运动伪影易受影响，其在动态和体积测量中的应用受限。本文介绍超快光学相干弹性成像（ultrafast OCE），这是一种用于体内实时体积生物力学成像的通用框架。通过将同步多相位采集与对频谱混叠具有固有鲁棒性的解调策略相结合，ultrafast OCE 将力学激励与采集速度解耦，仅需三张连续的 B 模式图像即可重建完整波场。该方法的帧率比传统方法高两个数量级，同时在从声学到超声范围的频率上保持高灵敏度。我们还开发了一种运动校正策略，可补偿生理条件下的整体组织运动。我们在动态拉伸的仿体和搏动的动脉中验证了 ultrafast OCE，并实现了角膜和皮肤的亚秒级体内体积成像。ultrafast OCE 可跨时空实时探查组织生物力学特性，为机械生物学、心血管研究和临床诊断开辟新机遇。
英文摘要
Imaging the mechanical properties of biological tissues in vivo with high spatial and temporal resolution is essential for understanding physiological function and disease progression. Optical coherence elastography (OCE) provides label-free, micrometer-scale mapping of tissue biomechanics, but its application to dynamic and volumetric measurements has been limited by slow acquisition speeds and susceptibility to motion artifacts. Here we introduce ultrafast optical coherence elastography (ultrafast OCE), a general framework for real-time volumetric biomechanical imaging in vivo. By combining synchronized multi-phase acquisition with a demodulation strategy intrinsically robust to spectral aliasing, ultrafast OCE decouples mechanical excitation from acquisition speed, enabling reconstruction of full wave fields from only three sequential B-mode images. The method achieves frame rates up to two orders of magnitude higher than conventional approaches while preserving high sensitivity over frequencies ranging from the acoustic to ultrasonic regimes. We further develop a motion-correction strategy that compensates for bulk tissue motion under physiological conditions. We validate ultrafast OCE in dynamically stretched phantoms and pulsatile arteries and demonstrate sub-second volumetric imaging of the cornea and skin in vivo. Ultrafast OCE enables real-time interrogation of tissue biomechanics across space and time, opening new opportunities for mechanobiology, cardiovascular research, and clinical diagnostics.
