AI 中文总结
本研究构建整合活神经元细胞的生物力学全身替代模型，结合实验与OpenSim模拟，揭示头部冲击参数与SH-SY5Y细胞反应的关联，为关联替代模型测量与人体头颈冲击响应提供基础。
AI 中文摘要
本研究开发了一种新型整合框架，利用整合活神经元细胞的生物力学全身替代模型，将冲击响应与细胞动力学关联起来。通过让该替代模型从30度、60度和90度的受控坐姿释放角度下落来模拟冲击事件，在市售替代模型的头部内放置三个垂直堆叠的细胞培养培养皿，每个培养皿中含有活的SH-SY5Y神经母细胞瘤细胞。使用六个加速度计的加速度测量值评估冲击事件的动态响应，其中三个传感器安装在头部表面，三个与细胞堆叠串联嵌入，同时测量头部模型的下落和变形运动学。并行使用基于OpenSim的修改后的肌肉骨骼模型模拟下落实验。研究发现，接触刚度的变化会导致模拟中预测的头部加速度产生最大变化。将细胞反应与测量的加速度进行比较时，氧化应激和细胞活力表现出与区域加速度和下落角度一致的趋势。在90度下落时，中位峰值线性加速度范围为170-258g，头部模型最大变形约为9.4mm，氧化应激增加至对照样本的约两倍。还对SH-SY5Y细胞的细胞漂移进行了量化，发现在90度冲击条件下，细胞漂移具有局灶性。还在OpenSim中模拟了相应的下落场景，并开发了初步校准关系，以比较物理替代模型和肌肉骨骼模型的运动学响应。最后，该框架为将实验替代模型测量值与冲击期间的人头颈响应关联提供了基础。
英文摘要
In this study, we develop a novel integrated framework that links the impact response with cellular dynamics using a live-neuron cell-integrated biomechanical full-body surrogate model. The impact event is simulated by allowing the surrogate model to fall from controlled seated release angles of 30-degree, 60-degree, and 90-degree. Three vertically stacked cell-culture Petri dishes, each containing live SH-SY5Y neuroblastoma cells, were placed inside the head of a commercially available surrogate model. The dynamic response of the impact event was evaluated using acceleration measurements from six accelerometers, comprising three sensors mounted on the head surface and three embedded in series with the cell stacks, along with kinematic measurements of the fall and deformation of the head model. In parallel, an OpenSim-based modified musculoskeletal model was used to simulate the fall experiment. We found that variation in contact stiffness produced the largest change in the predicted head acceleration in the simulation. When the cellular response and the measured accelerations are compared, oxidative stress and cell viability showed trends consistent with the regional acceleration and angle of fall. At the 90-degree fall, where median peak linear accelerations ranged from 170-258g, and the maximum headform deformation was approximately 9.4 mm, oxidative stress increased to approximately twice that of the control sample. We also quantified the cellular drift of SH-SY5Y cells, which is focal in nature for the 90-degree impact condition. The corresponding fall scenarios were also simulated in OpenSim and a preliminary calibration relationship was developed to compare the kinematic responses of the physical surrogate and musculoskeletal model. Finally, the framework provides a basis for relating experimental surrogate measurements to human head-neck response during impact.
